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題 名 | 芭樂籽乙醇萃取物對小鼠腹腔巨噬細胞細胞激素分泌之影響=Effect of Ethanol Extracts of Guava Seeds on Cytokine Secretions Using Mouse Peritoneal Macrophages in vitro |
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作 者 | 楊庭瑄; 林筱茜; 林金源; | 書刊名 | 農林學報 |
卷 期 | 62:2 2013.06[民102.06] |
頁 次 | 頁145-157 |
分類號 | 435.39 |
關鍵詞 | 抗發炎; 細胞激素; 乙醇萃取物; 芭樂籽; Anti-inflammation; Cytokine; Ethanol extract; Guava seed; |
語 文 | 中文(Chinese) |
中文摘要 | 先前研究已發現芭樂籽中含有免疫調節成分,但其有效成分難以被直接利用,因此本研究以80%的乙醇萃取其可能的有效成份,所獲得之芭樂籽乙醇萃取物在體外試驗以小鼠腹腔巨噬細胞進行免疫調節功能評估,樣品先進行細胞毒性試驗,取得非細胞毒性之最適作用濃度後,再以其最適作用濃度與小鼠腹腔巨噬細胞共同培養,探討單獨添加樣品或在脂多醣(lipopolysaccharide,LPS)存在下對小鼠腹腔巨噬細胞分泌細胞激素的影響。結果發現,乙醇萃取物在濃度4 – 250μg/ml時對細胞不具毒性,取此無細胞毒性濃度進行細胞激素分泌實驗,發現單獨添加樣品時,巨噬細胞分泌之促發炎(TNF-α、IL-6)或抗發炎細胞激素(IL-10),皆隨添加劑量的增加而顯著(P < 0.05)增加其分泌量,且呈現劑量反應關係,進一步分析其促發炎/抗發炎細胞激素分泌比值,亦有顯著增加的趨勢,推測芭樂籽乙醇萃取物可活化巨噬細胞而誘發其輕微的自發性發炎反應。在脂多醣存在下,腹腔巨噬細胞隨著樣品濃度的增加,其促發炎/抗發炎細胞激素分泌比值有顯著下降的趨勢,顯示芭樂籽乙醇萃取物可抑制脂多醣誘導的發炎作用。綜合本實驗研究結果,顯示芭樂籽乙醇萃取物具有免疫調節生物活性,可活化巨噬細胞及抑制脂多醣所誘發的發炎作用。 |
英文摘要 | In our preliminary study we found that there are immunomodulatory components in guava seeds, however effective components in guava seeds are difficult to be ingested directly. To obtain effective components and evaluate their possible immunomodulatory functions, guava seeds were extracted with 80% alcohol in this study. The isolated ethanol extracts of guava seeds (EEGS) were subjected to cytotoxic effect assay using mouse peritoneal macrophages to achieve optimal non-cytotoxic concentrations. The adopted optimal concentrations were cultured with mouse peritoneal macrophages in the absence or presence of lipopolysaccharide (LPS) for evaluating the effect of EEGS on cytokine secretions. The results showed that EEGS administered from 4 to 250μg/ml had not apparent cytotoxic effects on peritoneal macrophages, indicating optimal concentrations to the cells. The optimal concentrations were further administered to mouse peritoneal macrophages to assay cytokine secretion profiles. In the absence of LPS, both pro-inflammatory cytokines (tumor necrosis factor (TNF)-α and interleukin (IL)-6 and an anti-inflammatory cytokine (IL-10) secretion levels significantly (P < 0.05) increased by EEGS dose-dependently. Pro-/anti-inflammatory cytokine secretion ratios by the cells also increased dose-dependently, suggesting that EEGS might activate macrophages and slightly induce spontaneous inflammation. In the presence of LPS, the addition of EEGS significantly decreased pro-/anti-inflammatory cytokine secretion ratio by peritoneal macrophages dose-dependently, suggesting that EEGS might inhibit LPS-induced inflammation in macrophages. In conclusion, we found EEGS possessed immunomodulatory activities. EEGS alone might activate macrophages, but it might inhibit LPS-induced inflammation in macrophages. |
本系統中英文摘要資訊取自各篇刊載內容。