查詢結果分析
相關文獻
- 芭樂籽溶劑萃取物對小鼠初代脾臟細胞細胞激素分泌之影響
- 蟹殼甲殼素對小鼠脾臟細胞受脂多醣刺激分泌細胞激素之影響
- 中藥對過敏性鼻炎治療之免疫機轉研究
- 急性躁期患者細胞免疫功能之活化
- 氣功外氣對人類早期單核細胞株和流行性感冒病毒的效應
- 等速肌力訓練對細胞激素IL-6和IFN-γ的影響
- 細胞激素在急性胰臟炎的病因學上扮演的角色
- Excessive Expression of the Tumor Necrosis Factor-α Gene in the Kidneys of Patients with Membranous Glomerulonephritis
- TGF-ß在迷你豬牙周組織實驗性炎症中之含量變化
- Modulation of Immune Responses to DNA Vaccines by Codelivery of Cytokine Genes
頁籤選單縮合
題名 | 芭樂籽溶劑萃取物對小鼠初代脾臟細胞細胞激素分泌之影響=Effects of Guava Seed Solvent Extracts on Cytokine Secretions by Murine Primary Splenocytes |
---|---|
作者 | 連思惠; 顧琪玫; 林金源; | 書刊名 | 臺灣農業化學與食品科學 |
卷期 | 50:5/6 2012.10-12[民101.10-12] |
頁次 | 頁271-280 |
分類號 | 435.39 |
關鍵詞 | 細胞激素; 芭樂籽萃取物; 脾臟細胞; Cytokines; Extracts of guava seeds; Splenocytes; |
語文 | 中文(Chinese) |
中文摘要 | 為了解芭樂籽之免疫調節潛力,本研究以購入之芭樂果實收集其種子進行試驗。先將芭樂籽在40℃烘箱中乾燥後,再將芭樂籽磨成粉末,並將芭樂籽粉末分別加入四倍去離子水、80%乙醇或正己烷進行萃取,萃取液減壓濃縮去除有機溶劑後冷凍乾燥,取得芭樂籽的水、80%乙醇和正己烷萃取物,以分析其對小鼠初代脾臟細胞細胞激素分祕之影響。試驗結果顯示,水及80%乙醇萃取物在156、625及2,500μg/mL等濃度,正己烷萃取物在78、156及312μg/mL等濃度,對脾臟細胞無顯著細胞毒性:取樣品(非細胞毒性濃度)與脾臟細胞共同培養48小時,分析脾臟細胞抗發炎細胞激素介白質(interleukin, IL)-10和促發炎細胞激素IL-1β、IL-6及腫瘤壞死因子-a(TNF-a)分泌量的變化,顯示添加芭樂籽水萃取物有增加BALB/c雌鼠脾臟細胞分泌細胞激素之能力,具有免疫刺激活性:添加80%乙醇萃取物,則同時降低抗發炎和促發炎細胞激素的分泌,推測具有抗發炎及免疫抑制作用;正己烷萃取物則未見免疫調節潛力,綜合本研究結果,證實芭樂籽的水萃取物及80%乙醇萃取物在動物體外試驗具有不同的免疫調節活性,將來或可應用於不同的免疫調節目的。 |
英文摘要 | To understand whether guava seeds have immunomodulatory potential, guava (Psidium guajava L.) fruit was purchased and its seeds were isolated for study. Isolated guava seeds were dried in an oven at 40℃ and then milled into powder. The powder samples were extracted using four volumes of de-ionized water, 80% ethyl alcohol or n-hexane. The organic solvent in the filtrates were evaporated using a rotary evaporator and freeze dried to obtain guava seed water extracts (GSWE), eighty percent ethyl alcohol guava seed extracts (AGSE) and n-hexane guava seed extracts (HGSE), which were used to assess their effects on cytokine secretions by murine primary splenocytes in vitro. The results showed that GSWE and AGSE at 156, 625 and 2,500 g/mL, as well as HGSE at 78, 156 and 312 pg/mL did not significantly affect cell viability, reflecting their optimal non-cytotoxic concentrations to splenocytes. The apparent non-cytotoxic doses of GSWE, AGSE and HGSE were selected to treat splenocytes for 48 hours. Anti-inflammatory cytokine interleukin (IL)-10 and pro-inflammatory cytokines levels including IL-1β, IL-6 and tumor necrosis factor alpha (TNF-a) in the cell culture supernatants were determined. GSWE addition increased cytokine secretions by splenocytes, suggesting that GSWE might have an immunostimulatory activity. AGSE addition decreased both anti- inflammatory and pro-inflammatory cytokines, suggesting that AGSE might have anti-inflammatory and immunosuppressive effects. HGSE addition did not significantly affect splenocyte cytokine secretion profiles, suggesting that HGSE had little immunomodulatory potential. In conclusion, this study provides evidence that GSWE and AGSE exhibit differential immunomodulatory activities in vitro. Our results suggest that GSWE and AGSE may be of value for different immunomodulatory purposes in the future. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。