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| 題 名 | 運動預處理可能減少血漿對離體紅血球脂質氧化作用=Exhausted Exercise Preconditioning May Cause Plasma to Prevent Lipid Peroxidation of RBC in Vitro |
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| 作 者 | 沙部.魯比; 林嘉志; 楊忠祥; | 書刊名 | 文化體育學刊 |
| 卷 期 | 16 2013.06[民102.06] |
| 頁 次 | 頁53-68 |
| 分類號 | 528.9012 |
| 關鍵詞 | 氧化傷害; 抗氧化酶活性; 尿酸; Oxidative damage; Antioxidant enzyme activity; Uric acid; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 研究目的:運動預處理已知可減少接續激烈運動引起的氧化傷害,本研究擬探討力竭運動預處理,對紅血球受低氧再充氧處理的影響。方法:研究對象為8位自願參加研究的健康男性(年齡:28.9±8.3 yr,身高:170.1±5.9 cm,體重:68.9±7.4 kg)。採集漸進式運動負荷(graded exercise testing, GXT)跑步前(resting, r)、後(post exercise, e)血液立即離心,分為安靜紅血球(rR)、安靜血漿(rP)、運動紅血球(eR)與運動血漿(eP),並以磷酸緩衝溶液(PBS)重新組合後,比較rWB(r全血)、rRrP(r紅血球+r血漿)、rReP(r紅血球+e血漿)、rRs(r紅血球+磷酸緩衝溶液)。分別處理於低氧(hypobaric hypoxia, PO2=45 mmHg, H)或常氧(normbaric normoxia, PO2=152 mmHg, N)1hr後,再恢復常氧5分鐘(reoxgenation, N)。分析紅血球抗氧化酶活性、脂質氧化傷害指標(TBARS)與上清液尿酸、乳酸與葡萄糖濃度。結果:本研究發現運動預處理後,血漿乳酸(15.26±0.73 mM vs. 2.88±0.19 mM, p < 05)、葡萄糖(120.18±6.40 mg.dL^(-1) vs. 98.12±5.09 mg.dL^(-1), p < .05)與尿酸濃度(5.64±0.53 mg.dL^(-1) vs. 4.56±0.48 mg.dL^(-1), p < .05)顯著高於運動前。血液樣本rReP含有運動血漿(eP),其紅血球TBARS無顯著增加,但rWB、rRrP、rRs於N/N無含有運動血漿(eP),紅血球TBARS濃度顯著高於運動前(r)(146.35±32.48 nmol.gHb^(-1), 141.97±34.4 nmol.gHb^(-1), 148.42±29.8 nmol.gHb^(-1) vs. 59.72±16.31 nmol.gHb^(-1), p < .05)與運動預處理(e)(146.35±32.48 nmol.gHb^(-1), 141.97±34.4 nmol.gHb^(-1), 148.42±29.8 nmol.gHb^(-1), vs. 50.53±21.33 nmol.gHb^(-1), p < .05),表示運動後血漿可能具有防止脂質氧化作用。然而這種保護效果無法表現於H/N處理。在N/N或H/N處理的抗氧化酶,包括CAT、SOD、GPX活性在組別間比較均無顯著差異。結論:運動預處理血漿,可能減少離體紅血球的脂質氧化傷害,但無法減少低氧再充氧的傷害。這種保護作用可能屬於一種激效作用,是否與力竭運動後有較高的尿酸有關尚待進一步探討。 |
| 英文摘要 | Purpose: Exercise preconditioning has been known to reduce strenuous exercise-induced oxidative damages. To understand the underlining mechanisms, plasma samples isolated from exhausted exercises were used to investigate the possible protection effect on RBC with or without following hypoxia/reoxygenation. Methods: Eight healthy male participants were recruited voluntarily (age: 28.9 ± 8.3 yr, height: 170.1 ± 5.9 cm, weight: 68.9 ± 7.4 kg). Blood samples were collected before (resting, r) and after (post exercise, e) running by Bruce graded exercise testing (GXT). Plasma and RBC were separated and then recombined again into 4 groups: rWB (rWhole Blood), rRrP (rRBC + rPlasma), rReP (rRBC + ePlasma), rRs (rRBC + PBS). All groups were then treated with hypobaric hypoxia at PO2 = 45 mmHg (H/N) or normbaric normoxia at PO2 = 152 mmHg (N/N) for 1 hr and then recovered by N/N for 5 min. Data were to compare differences among the groups at indicators including antioxidant enzyme activities, peroxidized lipid (TBARS), lactate, glucose and uric acid. Results: As compared to the control group, plasma with exercise preconditioning had significantly increased levels of lactate (15.26 ± 0.73 mM vs. 2.88 ± 0.19 mM, p < .05), glucose (120.18 ± 6.40 mg.dL^(-1) vs. 98.12 ± 5.09 mg.dL^(-1), p <. 05) and uric acid (5.64 ± 0.53 mg.dL^(-1) vs. 4.56 ± 0.48 mg.dL^(-1), p < .05). Except for eReP, RBC's TBARS level was significantly elevated in rWB, rRrP, rRs after N/N treatment as compared to rP (146.35 ± 32.48 mnol.gHb^(-1), 141.97 ± 34.4 mnol.gHb^(-1), 148.42 ± 29.8 mnol.gHb^(-1) vs. 59.72 ± 16.31 mnol.gHb^(-1), < .05) or eP along (146.35 ± 32.48 mnol.gHb^(-1), 141.97 ± 34.4 mnol.gHb^(-1), 148.42 ± 29.8 mnol.gHb^(-1), vs. 50.53 ± 21.33 mnol.gHb^(-1), p< .05). However, this protection effect contributed to eP cannot be observed in groups after H/ treatment. All antioxidant enzyme activities among the groups were not different in both N/N and H/N treatments. Conclusion: Plasma with exhausted exercise preconditioning may reduce RBC peroxidized lipid damages but may not in hypoxia and reoxygenation. Further studies are needed to explore the role of uric acid in this reaction. |
本系統中英文摘要資訊取自各篇刊載內容。