頁籤選單縮合
題名 | 未經冷馴化之草莓莖頂超低溫冷凍保存研究=Study on the Cryopreservation of Non-cold Hardening Fragaria × ananassa Duch. Shoot Tips |
---|---|
作者 | 蕭翌柱; 陳冠文; | 書刊名 | 臺灣農業研究 |
卷期 | 63:1 2014.03[民103.03] |
頁次 | 頁57-67 |
分類號 | 435.365 |
關鍵詞 | 草莓; 超低溫冷凍保存; 填充溶液; 滲透壓保護; 植物玻璃質化溶液2; Fragaria × ananassa Duch.; Cryopreservation; Loading solution; Osmoprotection; Plant Vitrification Solution 2; |
語文 | 中文(Chinese) |
中文摘要 | 草莓(Fragaria × ananassa Duch.)屬於薔薇科(Rosaceae)多年生高價值經濟作物,並且有超過70個以上的國家進行栽培與量產,因此進行種原的長期保存益顯重要。本試驗的主要目的,在於建立未經冷馴化草莓(「桃園1號」)最適宜的超低溫冷凍保存處理方法。培植體在尚未浸入液態氮前的預處理結果如下:(1)莖頂培植體(1-2 mm^3)預培養於添加0.3 M蔗糖之MS基本鹽類培養基14 d後的平均存活率為100%;(2)將填充溶液(LS)中的蔗糖濃度自0.6 M提升至1.6 M並且滲透壓保護90 min,莖頂的平均存活率仍可達到100%;以及(3)使用植物玻璃質化溶液2(PVS2)脫水60 min後之莖頂平均存活率達到96.7% ± 4.7%,此百分比顯著高於PVS2脫水180 min(60.0% ± 14.1%)和240 min(46.7% ± 17.0%)的處理組。經PVS2脫水後,每10顆含莖頂的藻膠球及0.3 mL新鮮的PVS2被置入1個2 mL容量的抗凍管中,然後直接投進-196℃的液態氮至少保存1 h,經液態氮凍存後的抗凍管,在38℃恆溫水槽中迅速回溫60 s,此種含有莖頂的藻膠球繼代培養14 d後的再生率可達到80%。本研究各項試驗結果,將有助於建立及簡化草莓超低溫冷凍保存的程序。 |
英文摘要 | Strawberry (Fragaria × ananassa Duch.) is a high economic value perennial crop. It belongs to the Rosaceae family and is distributed in more than 70 countries. Therefore, the research on long-term storage of germplasm is important. The major objective of this study was to establish an optimum cryopreservation method of non-cold hardening Fragaria × ananassa Duch. cv. 'Taoyuan No.1'. The results obtained on pretreatment of explants prior to plunge into liquid nitrogen (LN) were as follows: (1) The survival rate of shoot tips (1-2 mm^3) was 100% when explants precultured on MS basal medium supplemented with 0.3 M sucrose for 14 d. (2) To increase sucrose levels from 0.6 M to 1.6 M in loading solution (LS) and then osmoprotected for 90 min, the survival rate of shoot tips remained 100%. (3) Survival rate of shoot tips achieved 96.7% ± 4.7% when treated with Plant Vitrification Solution 2 (PVS2) dehydration for 60 min. This percentage was significantly higher than treatments of PVS2 dehydration for 180 min (60.0% ± 14.1%) and 240 min (46.7% ± 17.0%). After PVS2 dehydration treatment, 10 shoot tip beads with 0.3 mL fresh PVS2 were transferred to a 2 mL cryotube and then plunged directly into LN for at least 1 h. After storage in LN, cryotubes were rapidly rewarmed in a water bath at 38℃ for 30 s. The final average regeneration rate of shoot tip beads (80%) was achieved after subcultured for 14 d. These research results appear promising for establishing and simplifying cryopreservation procedure of strawberry. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。