查詢結果分析
相關文獻
- 糖的利用在水稻種子發芽及子葉鞘伸長之淹水耐受性上扮演重要的角色
- 水稻澱粉水解酵素(αAmy3基因)表現於水稻幼苗中受到淹水的誘導及有氧的抑制
- 高粱孕穗期淹水對葉片糖類代謝的影響
- Sucrose-Starvation-Induced Changes in Polyamine and Abscisic Acid Levels of Suspension-Cultured Rice Cells
- 蔗糖、光照及溫度對水稻白化苗蔗糖合成酶異構酶含量之影響
- 高濃度蔗糖處理誘導水稻癒合組織再生植株過程中蛋白質圖譜之變化
- 高粱孕穗期淹水對子粒生長的影響
- 高蔗糖濃度促進水稻癒合組織再生植株之探討
- 水稻秧苗中合成蔗果三糖的酵素活性
- Effects of Waterlogging on Seed Germination, Electric Conductivity of Seed Leakage and Developments of Hypocotyl and Radicle in Sudangrass
頁籤選單縮合
題名 | 糖的利用在水稻種子發芽及子葉鞘伸長之淹水耐受性上扮演重要的角色=Sugar Availability Plays an Important Role in Tolerance to Flooding during Seed Germination and Coleoptile Elongation in Rice |
---|---|
作者 | 李勇成; 高銓宏; 陳鵬文; | 書刊名 | 嘉大農林學報 |
卷期 | 11:1 2014.02[民103.02] |
頁次 | 頁75-87 |
分類號 | 434.113 |
關鍵詞 | α-澱粉水解酵素; 種子發芽; 子葉鞘伸長; 淹水; 糖; 水稻; α-amylase; Seed germination; Coleoptile elongation; Flooding; Sugar; Rice; |
語文 | 中文(Chinese) |
中文摘要 | 大多數的穀類作物無法忍受土壤淹水所造成的傷害,而且在淹水的環境下種子也無法發芽。但是水稻卻具有很高的淹水耐受性,在淹水下種子可以發芽並且促進子葉鞘伸長與生長。水稻是唯一在種子淹水發芽時期會生成α-澱粉水解酵素的穀類作物,並提供可利用的糖作為能量來源,是水稻呈現淹水耐受性的重要因素之一。本研究比較不同的水稻品系,發現淹水下子葉鞘的伸長在品系間存在很大的差異。日本型水稻比印度型水稻在淹水下有較長的子葉鞘生長,而且水稻次基因家族三(subfamily 3)的α-澱粉水解酵素基因群在胚乳的表現量,在日本型品系日本晴中也遠高於印度型品系FR13A。我們發現FR13A種子在淹水下子葉鞘的伸長與生長明顯的受到糖的影響,但是對於日本晴則影響不顯著。我們接著利用基因轉殖水稻及RT-PCR方法分析α-澱粉水解酵素基因啟動子在水稻種子的糖及低氧反應。我們的結果顯示αAmy3啟動子在水稻種子的表現活性顯著的受到缺糖及低氧的誘導,而且糖抑制αAmy3基因表現凌駕於受到低氧的誘導表現。我們的結果證實在水稻種子發芽時期,αAmy3基因表現雖然受到低氧訊息調控,但是主要還是受到糖的訊息調控。因此推測儲存澱粉的分解及糖的利用在種子淹水發芽時期,可能扮演較為關鍵的角色。 |
英文摘要 | Most crops are not able to withstand soil flooding and their seeds fail to germinate under submerged conditions; in contrast, rice is known to be highly tolerant to flooding and could germinate and elongate the coleoptile. Rice is the only cereal that has the ability to produce α-amylase and has been proposed to be essential for carbohydrate metabolism for anaerobic germination and seedling growth. In the present study, our result s showed that coleoptile from different rice cultivars differ greatly in rates of elongation. In general, submergence-stimulated elongation of coleoptile in japonica cultivars is higher than that in indica cultivar and the rice subfamily 3 α-amylase gene s are higher expressed in hypoxic endosperms in japonica cultivar Nipponbare compared to that in indica cultivar FR13A. We found that under water, exogenous sugars significantly enhanced the elongation of coleoptile during seed germination in FR13A but not in Nipponbare. The transgenic approach and RT-PCR analysis were then employed to evaluate the response to sugar and hypoxia in terms of promoter activity of α-amylase genes during the anaerobic germination of rice. Our result showed that the expression of αAmy3 promoter is strongly induced by sugar starvation and hypoxia in geminated rice seed. We found that sugar repression overcomes hypoxia activation of αAmy3 expression in rice seed. Our results demonstrated that the expression of αAmy3 gene is regulated more significantly through sugar than through hypoxia signal in rice seed. Consequently, mobilization of starchy endosperm to provide available sugar is likely play the most important factor contributing to the anaerobic germination and seedling growth in rice. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。