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題名 | 以細胞模式篩選可能抑制骨質疏鬆的植株萃取物=Screening Potential Plant Extracts to Prevent Osteoporosis Using a Cell Model |
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作者 | 李互暉; 廖芸郁; 蔡依錦; 劉奎均; 陳立耿; 鄭可大; | 書刊名 | 嘉大農林學報 |
卷期 | 11:1 2014.02[民103.02] |
頁次 | 頁65-74 |
分類號 | 414.5 |
關鍵詞 | 植物萃取物; 骨質疏鬆; 成骨前驅細胞; 成骨細胞礦化; 骨化作用; plant extract; Osteoporosis; MC3T3-E1; Osteoblastic mineralization; Osteoblastic differentiation; |
語文 | 中文(Chinese) |
中文摘要 | 我們以一株小鼠的成骨前驅細胞MC3T3-E1的鈣化(礦化)情形來對120個植物萃取物進行篩選,找出可能抑制骨質疏鬆症的治療替代物。經過三重覆的篩選,我們篩選出兩株植物的萃取物,神秘果Synsepalum dulcificum(以下簡稱SD)與薄葉風藤Piper sintenense(以下簡稱PS)為最有潛力促進骨質礦化的萃取物。當在骨細胞分化實驗中添加SD或PS萃取物,從第七天開始,我們可觀察到額外添加任何其中一種植物萃取物0.05mg/ml以上濃度均造成細胞外型的變化,並逐漸有疑似細胞外骨化基質的累積。經過Alizarin Red-S染色含鈣基質顯示在SD及PS添加後確實促進鈣質的沈積。而進一步分析骨化作用重要標幟基因骨鈣素(osteoclacin, OCN)及骨橋蛋白(osteopontin, OPN)的表現,也証實添加的SD及PS時能夠促進成骨細胞的成熟,並且此種促進作用會隨著萃取物濃度的增加而加強。綜合以上結果,SD與PS的萃取物應具有抗骨質疏鬆的潛能。 |
英文摘要 | We attempt to screen candidate plant extracts that are able to prevent osteoporosis. We employed a preosteoblastic cell line, MC3T3-E1 for the experimental target. MC3T3-E1 can be calcified when is treated with vitamin C (ascorbic acid) and symbol-glycerophosphate for 21 days, and we suggested that this calcification may be enhanced by extra-supplies with some plant extracts. After screening more than 120 plant extracts, we acquired two extracts from Synsepalum dulcificum (SD) and Piper sintenense (PS) that appeared high potential to enhance mineralization. When cells were cultured in differentiation medium supplemented with more than 0.05mg/ml of either one extract, cell secreting cellular matrices was enhanced. Using Alizarin red-s staining, these matrices were verified abundant of calcium deposits. Staining results indicated that culture medium supplemented with 0.02mg/ml or above concentration of either one extract strongly enhanced osteoblastic mineralization in comparison of control. Moreover, the expressions of osteoblastic differentiation markers, osteocalcin(OPN) and osteopontin(OPN) were increased when MC3T3-E1 cells were stimulated with plant extracts. Also, the gene expression was increased in proportion to the concentration of plant extracts. We therefore conclude that two extracts from SD and PS are potential to treat osteoporosis. |
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