查詢結果分析
相關文獻
- 感染天竺葵之胡瓜嵌紋病毒之鑑定與特性分析
- C-jun Expression in Patients With Parkinson's Disease
- Genetic Variation of Recent Infectious Bronchitis Viruses Isolated in Taiwan
- 應用單管多引子反轉錄聚合酶連鎖反應檢測並區分家禽流行性感冒及新城雞病
- Complementary DNA Cloning and Analysis of Gene Structure of Pyruvate Kinase from Drosophila Melanogaster
- 應用聚合酶連鎖反應及核酸探針雜配法偵測齒舌蘭輪斑病毒
- Differential Display of mRNA in Human Lung Tissue and in Non-Small Cell Lung Cancer: A Preliminary Report
- 反轉錄聚合酶連鎖反應簡介及應用區分家禽流行性感冒及新城雞病
- The Preparation of RNA-Dependent RNA Polymerase Complex from Virus Infected Plants
- 菜豆黃化嵌紋病毒及胡瓜嵌紋病毒在唐菖蒲葉片之分佈及其對病毒檢測之影響
頁籤選單縮合
題 名 | 感染天竺葵之胡瓜嵌紋病毒之鑑定與特性分析=Identification and Molecular Characterization of Cucumber mosaic Virus Isolated from Pelargonium hortorum in Taiwan |
---|---|
作 者 | 吳沛育; 陳玉琪; 王惠亮; | 書刊名 | 植物病理學會刊 |
卷 期 | 22:2 2013.06[民102.06] |
頁 次 | 頁105-119 |
分類號 | 433.4 |
關鍵詞 | 天竺葵; 病徵; 反轉錄聚合酶連鎖反應; 胡瓜嵌紋病毒; 序列相同度; Geranium; Symptom; Cucumber mosaic virus; CMV; Sequence identity; |
語 文 | 中文(Chinese) |
中文摘要 | 天竺葵(Pelargonium hortorum)為牻牛兒苗科(Geraniaceae)、天竺葵屬(Pelargonium)之多年生宿根花卉,原產於非洲南部。花色多樣,花期極長,適合花壇或盆栽。2010年於彰化田尾發現葉片有明顯葉脈黃化(vein-clearing)及黃斑(chlorotic spots)病徵的天竺葵植株。經三次單斑分離後獲得一純系病毒分離株,稱為GE 1-3。初步經寄主範圍測試、電子顯微鏡觀察、血清學鑑定及利用胡瓜嵌紋病毒(Cucumber mosaic virus,CMV)之專一性引子對(CPr/CPf)進行反轉錄聚合酶連鎖反應(reverse-transcription polymerase chain reaction,RT-PCR),鑑定結果為CMV,暫稱其為CMVGE 1-3。CMV GE 1-3病毒全長RNA 1、RNA 2、RNA 3分別具有3,361、3,047及2,221個核苷酸。將CMV GE 1-3與National Center for Biotechnology Information(NCBI)網站登錄之其他亞群CMV序列進行比對發現:在RNA 3之5'端非轉譯區比較上,顯示與IB亞群相同度最高達98%,在鞘蛋白序列相同度比較上,與IB亞群義大利Tfn系統(Accession No. Y16926)及台灣NT 9系統(Accession No. D28780),核苷酸相同度達95%;胺基酸相同度與IB亞群義大利Tfn系統達100%,與台灣NT9系統達99%。綜上,證實感染天竺葵之CMV GE 1-3分離株為CMV的天竺葵分離株,且為IB亞群。本報告為台灣地區CMV感染天竺葵之首篇紀錄。 |
英文摘要 | Geranium (Pelargonium hortorum), a popular bed and potting plant, is a perennial ornamental of the family Geraniaceae oringinated from southern Africa. Geranium plants with chlorotic spots on leaves, which is a typical symptom of of viral infection, were collected at Tianwei, Changhua in 2010.A virus, with a diameter of about 28 nm, was isolated via three successive single lesion isolations on Chenopodium quinoa and was designated as GE 1-3. The preparations of purified GE 1-3 positively reacted with antiserum against Cucumber mosaic virus (CMV) in indirect enzyme-linked immunosorbent assay (indirect ELISA) indicating GE-13 is a CMV isolate. The relative molecular weight of the virus coat protein (CP) was estimated to be about 26.9 kDa by SDS-PAGE and the CP can be detected by the CMV antiserum in western blots. The nucleotide sequences of full-length genome of GE 1-3 have been determined. The genomic RNAs 1, 2 and 3 consist of 3361, 3047, 2221 nucleotides, respectively. Comparison of sequence identities based on the nucleotide and deduced amino acid sequences of individual genes among GE 1-3 and that of other documented CMV isoaltes indicating that the GE 1-3, the virus causing chlorotic spots on geranium leaves, is a CMV isolate of subgroup IB. The results of this study indicate that, this is the very first report of a subgroup ΙB CMV infects geranium plants. |
本系統中英文摘要資訊取自各篇刊載內容。