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題名 | 甜椒細菌性莖腐病之病原鑑定、偵測與藥劑篩選=Identification, Detection, and Bacteriocide Screening of Bacterial Stem Rot of Sweet Pepper |
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作者 | 蔡佳欣; 安寶貞; 陳美德; 申屠萱; 李昀珊; | 書刊名 | 植物保護學會會刊 |
卷期 | 55:1 2013.03[民102.03] |
頁次 | 頁1-10 |
分類號 | 435.27 |
關鍵詞 | 甜椒; 藥劑篩選; 細菌性莖腐病; 病原鑑定; Sweet pepper; Bacterial stem rot; Pectobacterium carotovorum subsp.; Carotovorum; Rapidly diagnosis; Bactericide screening; |
語文 | 中文(Chinese) |
英文摘要 | Stem rot disease of sweet pepper was found in Nantou and Yunlin counties in 2010.Water-soaked, longitudinal, and brown lesions were observed on the surface of stem, andthe pith showed necrotic and disintegrated symptoms. Bacteria isolated from the infectedtissues were verified to cause stem rot of sweet pepper in pathogenicity test. The pathogenwas further identified as Pectobacterium carotovorum subsp. carotovorum (Pcc) based onphysiological and chemical tests, Biolog identification, and 16S-23S rDNA intergenic spacerregion sequences. Polymerase chain reaction using the primer pair Ec3F/Ec4R was developedfor rapid diagnosis of Pcc. The 497-bp DNA fragment corresponding to Pcc can be amplifiedfrom the mechanically inoculated sweet pepper plants. The bactericide oxolinic acid has themost efficiency to inhibit Pcc growth. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。