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題名 | 病例報告:白肉種雞之馬立克病併發沙門氏桿菌感染症=Case Report: Marek's Disease Complicated with Salmonella Albany Infection in Broiler Breeders |
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作者 | 羅登源; 鄭如娟; 張銘煌; 劉景怡; 陳鍵程; 陳秋麟; 郭鴻志; Lo, Dan-yuan; Cheng, Ju-chuan; Chang, Ming-huang; Liu, Jing-yi; Chen, Jian-cheng; Chen, Chiou-lin; Kuo, Hung-chih; |
期刊 | 臺灣獸醫學雜誌 |
出版日期 | 20130900 |
卷期 | 39:3 2013.09[民102.09] |
頁次 | 頁182-187 |
分類號 | 437.717 |
語文 | chi |
關鍵詞 | 白肉種雞; 原位雜合反應; 馬立克病; 沙門氏桿菌; Broiler breeder; In situ hybridization; Marek's disease; Salmonella enterica; |
中文摘要 | 雲林縣某一白肉種雞場,總飼養數約3,000羽。於2012年3月中旬,該場17週齡雞隻出現雞冠蒼白及腳部麻痺僵直情形,發生率約2.3%,死亡率約0.03%。肉眼病變可見肝臟、脾臟與腎臟多發局部黃白色結節;細菌學檢查於肝臟分離出沙門氏桿菌;分子生物學檢查使用聚合酶鏈鎖反應針對血清一型馬立克病病毒之meq基因進行增幅,並透過核苷酸與胺基酸序列比對後結果為血清一型馬立克病;組織病理學檢查肝臟、脾臟、腎臟、腸道與坐骨神經等組織器官均可見淋巴球樣細胞浸潤,於脾臟切片針對meq基因進行原位雜合反應,結果可見陽性訊號出現於淋巴球樣細胞之細胞核內。因此,依據臨床症狀、肉眼病變與實驗室檢查結果,確診本病例為白肉種雞之馬立克病併發沙門氏桿菌感染症。 |
英文摘要 | The reported case happened at a 17-week-old, approximately 3000 broiler breeders farm in Yunlin County. The owner described that the chickens showed paralysis of both legs and pale comb on the middle of March, 2012. The morbidity and mortality of affecting chickens were 2.3% and 0.03%, respectively. The macroscopic lesions at liver, spleen and kidney included enlargement and multifocal yellow to white nodules. In microbiological examinations, bacteria isolated from liver tissues were identified as Salmonella enteric serovar Albany. Both PCR amplification and nucleotide sequence results for meq gene of Marek's disease provided the evidence of Marek's disease virus 1 infection. The histopathology showed that there were extensive and multifocal lymphoid cells infiltration in liver, spleen, kidney, intestine and sciatic nerve. In situ hybridization of the meq probe on spleen tissue sections also demonstrated the positive signals were in the nucleus of lymphoid cells. Based on all the aforementioned results, the final diagnosis of this case was Marek's disease complicated with Salmonella Albany infection in broiler breeders. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。