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相關文獻
- 穿心蓮乙酸乙酯層區分物對影響腸病毒感染力之研究
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題名 | 穿心蓮乙酸乙酯層區分物對影響腸病毒感染力之研究=Effect of Andrographis paniculata EtOAc Fraction Extract on Enterovirus 71 Infection |
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作者 | 林璧鳳; | 書刊名 | 中醫藥年報 |
卷期 | 29:8 2011.09[民100.09] |
頁次 | 頁487-513 |
專輯 | 中醫藥基因體相關研究 |
分類號 | 414.32 |
關鍵詞 | 穿心蓮; 腸病毒71型; Andrographis paniculata; EV71; 14-deoxy-11; 12-didehydroandrographolide; |
語文 | 中文(Chinese) |
中文摘要 | 自從1998 年來台灣多次發生腸病毒的感染與流行,尤以腸病毒71 型為甚,至今仍無有效藥物治療,且許多臨床症狀已知腸病毒71 型感染之病人,呈現嚴重發炎反應。腸病毒71 型(EV71),屬於小RNA 病毒科(Picornaviridae)中的腸病毒屬(Enterovirus),是腸病毒中最主要造成手足症以及神經方面嚴重併發症的病毒。穿心蓮(Andrographis paniculata (Burm. F.) Nees)為爵床科一年生草本植物的全草及葉,有清熱、解毒、抗病毒之效。為響應衛生署中草藥委員會所提出研 究類之「中草藥與感染正疾病」之研究重點,探討穿心蓮乙酸乙酯層區分物對腸病毒71 型之影響及其機轉,本實驗欲以分配萃取進行分離純化所得之穿心蓮乙酸乙酯層(EtOAc, EA)區分物,利用腸病毒易感染的細胞(Rhabdomyosarcoma, RD cell)作為腸病毒感染的標靶細胞(Target cell),進行細胞共同培養方式,探討穿心蓮及其各種萃取溶液中的乙酸乙酯層和進一步的區分物之作用,偵測是否減少腸病毒毒殺目標細胞能力,探討中草藥是否可阻斷腸病毒與標靶細胞之間的作用,並期能求得有效抑制腸病毒或甚至其他類似病毒的有效成份。本實驗已成功由穿心蓮乙酸乙酯萃取物分離、純化得到26 個化合物,將其與腸病毒培養,實驗結果發現5-hydroxy-7,8-dimethoxyflavanone (2)、Cinnamic acid (5)、5-hydroxy-7,8,2’,5’-tetramethoxyflavone (9)、andrograpanin (10)、ergosterol peroxide (11)、新化合物(15)、14-deoxy-11,12-didehydroandrographolide (17)、3,19-O-acetyl-14-deoxy-11,12-didehydroandrographolide (19)、新合成衍生物20、23、24、25 等化合物具保護腸病毒感染之潛力。 |
英文摘要 | Enterovirus 71 (EV71) belongs to Enterovirus genus within Picornaviridae. Enterovirus 71 has been the most important enterovirus to cause hand-foot and mouth disease accompanied with neurological complication. Andrographis paniculata (Burm. F.) Nees. (It is called Chuan-Chin-Lian in Chinese) of Acanthaceae family is one of the Chinese herbs reputed to be effective in the treatment of infection, inflammation, cold and fever. To investigate and pursue a effective Chinese herb medicine to for anti-infection, the effect of Andrographis paniculata EtOAc fraction extract will be studied using EV71 target cell RD cells for co-culture study of Andrographis paniculata. Total 12 pure compounds were isolated by the bioassay guided fractionation test. The isolated active compounds were identified by NMR and mass spectrometry to elucidate the chemical structures. Our data shown that 5-hydroxy-7, 8-dimethoxyflavanone (2), Cinnamic acid (5), 5-hydroxy-7,8,2’,5’ -tetramethoxyflavone (9), andrograpanin (10), ergosterol peroxide (11), compound 15 was for the first time isolated from A. paniculata and reported here as new natural products, 14-deoxy-11, 12-didehydroandrographolide (17), synthesized products 3,19-O-acetyl-14-deoxy-11, 12-didehydroandrographolide (19) and new synthesized products 20, 23, 24, 25 all have anti-EV71 infection in RD cell. |
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