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題 名 | 複合奈米粒子胰島素載體之研究=Hybrid Nanoparticle Insulin Carriers |
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作 者 | 彭麗靜; 黃克峰; 官常慶; 劉繼賢; | 書刊名 | 藥學雜誌 |
卷 期 | 28:3=112 2012.09[民101.09] |
頁 次 | 頁26-30 |
分類號 | 418.4 |
關鍵詞 | 經皮輸藥系統; 幾丁聚寡醣; 交聯劑; 胰島素; 奈米粒子; |
語 文 | 中文(Chinese) |
中文摘要 | 罹患糖尿病有1.8億人口之多而且大約每年有290萬人口死亡,死亡率預計未來十年增加50%1。由於傳統治療上給予胰島素皮下注射之不便,故嘗試多種的基質做為給予胰島素之傳遞載體,對於解決注射胰島素疼痛之替代方法都很有興趣。若用蛋白質治療,給予經皮輸藥是一個方便的方法,所以經皮路徑提供有利於胰島素傳遞之另一種非侵入性的途徑。本研究主要是以配製幾丁聚寡醣 (chitosan oligosaccharide;COS) /牛血清白蛋白 (bovine serum albumin;BSA) 複合製成奈米粒子當胰島素之載體,來探討奈米粒子包覆胰島素之效率與體外之經皮穿透量。而 BSA/COS 複合奈米粒子是併用交聯劑 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride (EDC),藉由油包水型 (W/O) 奈米乳劑系統以相轉移溫度程序 (phase inversion temperature procedure;PIT) 和離心的方法製備而成,且胰島素可藉由靜電力吸附在複合的奈米粒子中。我們發現4% BSA,2% COS 與2% EDC 在 pH 5.5 反應二小時,可得到理想的奈米粒子。且其包覆胰島素的效率 (EE) 是88.5%。此胰島素的奈米粒子載體也顯示了成功的經豬皮穿透量,和持續的釋藥,結果顯示此複合奈米粒子具有作為胰島素經皮傳遞之潛能。 |
英文摘要 | Diabetes mellitus (DM) is suffered by more than 180 million people and is responsible for approximately 2.9 million deaths each year. This mortality rate is expected to increase by 50% in the next decade1. Due to the inconvenience of the traditional treatment of diabetes by subcutaneous injection of insulin, various attempts are made in the vehicles for insulin delivery. Alternative painless methods to administer insulin are of great interest. Transdermal drug delivery is a convenient method for delivery of therapeutic protein since the transdermal route offers certain advantages among other non-invasive routes of insulin delivery. The aim of this study was to prepare the chitosan oligosaccharide (COS) and bovine serum albumin (BSA) hybrid nanoparticle for insulin delivery. The nanoparticle preparation, insulin encapsulation efficiency (EE) and in vitro skin permeation were systematically investigated. The BSA/COS hybrid nanoparticles were synthesized by using water-in-oil (W/O) nanoemulsion as the template and using 1-ethyl-3- (3-dimethyl aminopropyl) -carbodiimide hydrochloride (EDC) as the cross-linking agent. The hybrid nanoparticles were collected by using the phase inversion temperature procedure and centrifugation. Insulin could be adsorbed into the hybrid nanoparticles via electrostatic force. We found that the encapsulation efficiency of insulin on BSA/COS hybrid nanoparticles was 88.5%. The optimal procedure for nanoparticle preparation was 4% BSA, 2% COS and 2% EDC at a pH 5.5 condition reacted for 2 hours. The insulin-loaded nanoparticles showed a successful permeation through the porcine skin and the sustained release for insulin could be obtained. Our results highlighted the potentials of the hybrid nanoparticles for the insulin transdermal delivery. |
本系統中英文摘要資訊取自各篇刊載內容。