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題 名 | Production of Acteoside in Hairy-Root Culture of Paulownia fortunei Hemsl=培養泡桐毛狀根生產洋丁香酚 |
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作 者 | 古國隆; 許菁芬; 廖宇賡; | 書刊名 | 臺灣林業科學 |
卷 期 | 27:1 2012.03[民101.03] |
頁 次 | 頁13-29 |
分類號 | 436.259 |
關鍵詞 | 洋丁香酚苷; 毛狀根; 泡桐; 二次代謝物; 毛蕊花苷; Acteoside; AS; Hairy root; Paulownia fortunei; Secondary metabolite; Verbascoside; |
語 文 | 英文(English) |
中文摘要 | 試管內培養的泡桐(Paulownia fortunei Hemsl.)實生苗接種農桿根群菌 (Agrobacterium rhizogenes, BCRC 15011(ATCC 43057))之後可誘導毛狀根(hairy root)發生並生產洋丁香酚苷(acteoside, AS)。先從泡桐毛狀根群7-6、23-1及55-1號進行高產量篩選,其中以55-1號培養在MS液體培養基(50 mL flask^(-1)),有最高產量(13.12±2.36 mg flask^(-1))。後續以該根群進行之研究顯示,MS培養基或將其濃度稀釋1/2或1/4,皆能有效提供培養基中之營養成分供毛狀根生長並產製AS,且均優於受測之其它濃度(p < 0.05)。在不同蔗糖濃度影響AS生產的試驗中,含有60 g L^(-1)蔗糖的培養基促進了根群的生長,因此可獲得最佳的AS產量。乙醇(ethanol; EtOH)為配製多種誘引因子常用之溶劑,試驗顯示乙醇劑量達9.5%(v/v)時有抑制AS產量的結果。以適量的乙醇為溶劑配製甲基茉莉酸(methyl jasmonate; MeJA)(0~50 μM)成為誘引因子時,在根群培養至第28天時給予24 h的短暫刺激,對AS的生產並未形成顯著的影響。未來可將此誘引刺激的時間及濃度做進一步的調整,以改善從毛狀根生產AS的情形。 |
英文摘要 | In vitro grown paulownia (”Paulownia fortunei Hemsl.”) seedlings were inoculated with ”Agrobacterium rhizogenes” (BCRC 15011; (ATCC 43057)) to produce hairy roots for acteoside (AS) production. Hairy-root lines 7-6, 23-1, and 55-1 were first cultured in MS liquid medium (50 mL flask^(-1)) to monitor their AS production, and line 55-1 was determined to have the maximal productivity (13.12±2.36 mg flask^(-1)). Subsequent examinations of this culture line revealed that MS medium and its dilutions (×1/2 and ×1/4 strength) all provided suitable nutrient components for AS production and were better than the other concentrations tested (p < 0.05). It was observed that when the medium was supplemented with 60 g L^(-1) sucrose, better AS production was also achieved (p < 0.05) due to enhancement of root growth. Ethanol (EtOH; 9.5% (v/v)), a commonly used solvent for elicitor preparation, was determined to be inhibitory and caused a correspondingly low AS content when applied to the medium. A 24-h pulse treatment of methyl jasmonate (MeJA), dissolved in an appropriate concentration of EtOH and adjusted to a final concentration of 0~50 μM, was tested on a 28-d-old root culture and was noted to have had a non-significant effect on AS accumulation. Further investigation is necessary to define a better combination of pulse duration and elicitor concentration to improve AS production with this culture system. |
本系統中英文摘要資訊取自各篇刊載內容。