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頁籤選單縮合
| 題 名 | Authentication of Domestic Taiwan Rice Varieties Based on Fingerprinting Analysis of Microsatellite DNA Markers=利用微衛星DNA標誌鑑別臺灣市售米常見品種 |
|---|---|
| 作 者 | 莊雪玉; 盧虎生; 胡凱康; 張孟基; | 書刊名 | Botanical Studies |
| 卷 期 | 52:4 2011.10[民100.10] |
| 頁 次 | 頁393-405 |
| 分類號 | 434.111 |
| 關鍵詞 | DNA指紋分析; 微衛星標誌; 多型性; 水稻; 簡單重覆序列; DNA fingerprinting analysis; Microsatellite markers; Polymorphism; Principle component analysis; PCA; Rice; Simple sequence repeat; SSR; |
| 語 文 | 英文(English) |
| 中文摘要 | 為追溯、偵測並防止混米之目的,本研究利用微衛星標誌(SSR)來鑑別台灣常見市售國產米及國外進口食米品種。共使用36個國內外食米品種及32組微衛星標誌進行水稻多型性之DNA指紋分析。結果於32個基因座上共得到306個不同之等位基因。每個基因座可有3至21個片段,平均每個基因座為9.6個片段。多態性訊息含量Polymorphic information value (PIC),介於0.205(RM7023)至0.926 (RM333),平均為0.75。其中RM101, RM333, RM475, RM1387, RM1388, RM2835, RM5428及RM5704微衛星標誌可有效區別台灣國產米。經由主座標principle component analysis (PCA)及集群分析(clustering analysis),發現來自不同國家之食米品種可分為2群。最後為防範混米,本研究也建立了台灣良質米品種鑑定及純度檢測流程表。在實際檢測應用上,以不同比例之台稉九號及台稉十六號進行混米檢測,其檢出之靈敏度約為3% - 7.5%。 |
| 英文摘要 | Microsatellite marker (SSR) analysis was used to differentiate between domestic Taiwan rice varieties and foreign rice cultivars for authentication and traceability. A panel of 32 microsatellite DNA markers and 36 rice varieties from different countries were used for comparative polymorphism analysis. A total of 306 alleles were observed in 32 loci. The number of alleles per locus ranged from 3 to 21, with an average of 9.6. Polymorphic information content (PIC) values varied from 0.205 (RM7023) to 0.926 (RM333), with an average of 0.75. The most useful markers identified for efficient differentiation of domestic Taiwan rice identities were RM21, RM22, RM101, RM333, RM475, RM1387, RM5704, and RM7545. Principle component analysis (PCA) score plot and clustering analysis were sufficient to discriminate between two different groups of rice varieties based on geographic origins. To prevent fraudulent commercial activity, these results provide effective SSR marker sets and workflow for regular genotype verification and premium rice variety purity monitoring in Taiwan. Finally, to ensure the practical use of DNA fingerprinting technology in rice adulteration tests, sensitivity as low as 3 to 7.5 % can be detected by mixing different ratios of Taikeng 9 and Taikeng 16. |
本系統中英文摘要資訊取自各篇刊載內容。