查詢結果分析
相關文獻
- A One-Time Inducible Transposon for Terminating Selectable Markers in Transgenic Plants
- Fusion of the Transposase with a Classical Nuclear Localization Signal to Increase the Transposition Efficiency of Ac Transposon
- 轉基因作物應用於植物保護之現況
- Coat Protein-Mediated Resistance to Tomato Mosaic Virus in Systemic and Local Lesion Hosts
- 如何監測評估轉基因植物的生態風險
- 合成型蘇力菌毒素基因crylA(c)與野生型基因crylC之共同轉殖及其在菸草中殺蟲效力之表現差異
- 構築含無啟動子報導基因之可誘導轉位子
- Construction of the Binary Vector with Bi-Selectable Markers for Generating Marker-Free Transgenic Plants
- 脂質轉運蛋白與耐力運動表現的關係
- 轉基因策略發展雙生病毒抗性植物之現況及展望
頁籤選單縮合
題名 | A One-Time Inducible Transposon for Terminating Selectable Markers in Transgenic Plants=單次轉位之可誘導轉位子終止轉基因篩選標記 |
---|---|
作者姓名(中文) | 戴宏光; 李冠德; 常玉強; | 書刊名 | Botanical Studies |
卷期 | 52:4 2011.10[民100.10] |
頁次 | 頁375-381 |
分類號 | 434.28 |
關鍵詞 | 轉位酶; 可誘導轉位子; 篩選標記基因; 轉基因植物; Ac transposase; Epsps; Inducible transposon; Selectable marker; Transgenic plants; |
語文 | 英文(English) |
中文摘要 | 使用篩選標記基因(selectable marker genes)可以快速、有效篩選轉殖株,然而在獲得轉殖株後,篩選標記基因就無特殊用途,並有生物安全疑慮,本研究建構單次可誘導轉位子以終止篩選標記之功能。將轉位酶基因上游建構可誘導啓動子,且轉位子之5端構築於轉位酶顯子(exon)C和D間,而將轉位子之3端建構於抗嘉磷賽之epsps(5-enolpyruvylshikimate-3-phosphate synthase;篩選標記基因 ) 顯子1和2之間,完成之構築命名為COKC。透過誘導劑水楊酸處理,企圖使轉位子轉位(transposition) 將兩端及其內部分轉位酶基因和部分epsps基因片段切離,達到終止轉位酶基因和修飾之epsps基因之功能。 |
英文摘要 | Since the maize transposon Ac can relocate to a new location within the genome, it has been used in removing selectable markers in transgenic plants. We previously developed an inducible transposon system to truncate a selectable marker in transgenic plants by locating one end of the transposon in the intron of the marker gene (glyphosate tolerant epsps gene). We have now improved upon this system by locating the other end of the transposon in the intron of the transposase gene, which is controlled by the inducible promoter (PR-1a). Treatment with salicylic acid induced transposition of this transposon, COKC, which led to both marker gene and transposase gene breakages in exons. The behavior of COKC was analyzed in singlecopy transgenic rice plants. We determined the expression of the modified transposase and epsps genes and the transposition events in transgenic plants. The COKC element thus exhibits potential as a tool to create “marker-off” transgenic plants for woody or vegetatively propagated plants species. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。