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題 名 | 添加外源性一氧化氮、細胞激素與脂多醣體對吳郭魚頭腎來源巨噬細胞發生apoptosis之影響=The Effects of Nitric Oxide Donors, Cytokines, and Lipopolysaccharide on Apoptosis of the Tilapia Head Kidney-derived Macrophages |
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作 者 | 鄭清福; 洪紹文; 林育興; 劉邦成; 陳明輝; 涂青宇; 張鎮璿; 邱繡河; 王渭賢; | 書刊名 | 臺灣獸醫學雜誌 |
卷 期 | 37:3 2011.09[民100.09] |
頁 次 | 頁169-179 |
分類號 | 437.22 |
關鍵詞 | 細胞計畫性死亡; 細胞激素; 頭腎巨噬細胞; 脂多醣體; 一氧化氮; Apoptosis; Cytokine; Head kidney-derived macrophages; Lipopolysacharide; Nitric oxide; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究之目的在於探討一氧化氮(nitric oxide; NO)、細胞激素與脂多醣體(lipopolysacharide; LPS)對吳郭魚頭腎巨噬細胞免疫功能之影響,並嘗試以流式細胞儀(flow cytometer)來分析NO對頭腎巨噬細胞的影響。實驗結果發現頭腎巨噬細胞如分別以干擾素-γ(interferon-γ; IFN-γ)和LPS、腫瘤necrosis因子-α(tumor necrosis factor-α; TNF-α)及LPS與IFN-γ作用24小時後,均能誘發頭腎巨噬細胞發生apoptosis,同時發現IFN-γ亦可誘發頭腎巨噬細胞發生necrosis。然而,當添加低濃度的NO donors時,結果發現會抑制經IFN-γ與LPS作用過之頭腎巨噬細胞發生apoptosis,同時亦發現介白素-4(interleukin-4; IL-4)和介白素-10(interleukin-10; IL-10)均會抑制頭腎巨噬細胞發生apoptosis。另外,實驗結果顯示頭腎巨噬細胞經高濃度之NO donors誘導24小時後,頭腎巨噬細胞會發生apoptosis,並且其發生之比例會隨著NO donors的濃度的提高而增加。 |
英文摘要 | The aims of this investigation were to study the effects of nitric oxide donors, cytokines, and lipopolysaccharide (LPS) on the non-specific immune responses of the tilapia (Oreochromis hybrids) head kidney-derived macrophages by using flow cytometry. Our results showed that tilapia head kidney-derived macrophages treated with IFN-γ, TNF-α, and IFN-γ plus LPS for 24 h exhibited a very high percentage of apoptotic cells. At the same time, IFN-γ also induced apoptosis in tilapia head kidney-derived macrophages. However, low doses of nitric oxide donors were able to inhibit apoptosis in the cells. The percentage of apoptosis of the tilapia macrophages decreased with addition of IL-4 and IL-10. On the other hand, tilapia head kidney-derived macrophages treated with high doses of nitric oxide donors for 24 h expressed a very high percentage of apoptosis. Simultaneously, the effect seemed to be dose-dependent. |
本系統中英文摘要資訊取自各篇刊載內容。