查詢結果分析
相關文獻
- Resveratrol Treatment Attenuates the Wound-Induced Inflammation in Zebrafish Larvae through the Suppression of Myeloperoxidase Expression
- 免疫、抗發炎與代謝
- Apoptotic Cells Induce the Wound Healing via the Zebrafish Liver Cell Model
- 淨白成分抑制酪胺酸酶活性和斑馬魚胚胎黑色素生成之研究
- Polydatin from Radix Polygoni Multiflori Inhibits Inflammatory Response and Alveolar Bone Loss in Experimental Periodontitis in Rats
- 麻杏甘石湯對塵蟍誘發氣喘天竺鼠之影響
- Inflammatory Pseudotmor of the Lung: Report of a Case
- 火針針刺對家兔肌肉組織之影響
- 中醫望診(舌診)系統(2):舌紅外線影像攝影系統在上消化道病人之運用(2-2)
- Serum Hepatocyte Growth Factor Levels in Patients with Inflammatory Lung Diseases
頁籤選單縮合
題 名 | Resveratrol Treatment Attenuates the Wound-Induced Inflammation in Zebrafish Larvae through the Suppression of Myeloperoxidase Expression=白藜蘆醇減緩斑馬魚傷口引發發炎之作用機制 |
---|---|
作 者 | 廖勻楓; 邱閔絹; 蔡振寧; 溫啟仲; 王芸馨; 鄭建中; 陳曜鴻; | 書刊名 | Journal of Food and Drug Analysis |
卷 期 | 19:2 2011.06[民100.06] |
頁 次 | 頁167-173+241 |
分類號 | 414.5 |
關鍵詞 | 環氧合酶; 發炎; 骨髓過氧化酶; 白藜蘆醇; 斑馬魚; Cyclooxygenase; Inflammation; Myeloperoxidase; Resveratrol; Zebrafish; |
語 文 | 英文(English) |
英文摘要 | Resveratrol, a polyphenolic phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line “Tg (mpx:GFP)” expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and cyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was not affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dose-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in vivo evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level. |
本系統中英文摘要資訊取自各篇刊載內容。