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題 名 | The XopE2 Effector Protein of Xanthomonas campestris pv. vesicatoria is Involved in Virulence and in the Suppression of the Hypersensitive Response=茄科細菌性斑點病菌 XopE2 有效蛋白具有毒性與抑制過敏性反應的功能 |
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作 者 | 林榕華; 彭致文; 林元春; 彭慧玲; 黃秀珍; | 書刊名 | Botanical Studies |
卷 期 | 52:1 2011.01[民100.01] |
頁 次 | 頁55-72 |
分類號 | 435.271 |
關鍵詞 | 增幅限制片段核酸多型性分析; 茄科細菌性斑點病; 第三型分泌系統; XopE2蛋白; 過敏性反應; Amplified restriction fragment length polymorphism; Bacterial spot; Hypersensitive response; Type III secretion system; XopE2 effector; |
語 文 | 英文(English) |
中文摘要 | 由 Xanthomonas campestris pv. vesicatoria (Xcv) 引起的茄科細菌性斑點病,需透過第三型分泌系統的調控機制和其分泌的有效蛋白 (effectors) 引發其病原性。在此研究中,利用增幅限制片段核酸多型性技術 (AFLP) 調查台灣茄科植物細菌性斑點病菌的變異性,並選殖出一 xopE2的同源基因。本實驗中所採用的 14株菌株依據澱粉分解酶有無,可將菌株區分為 A、B兩菌群。根據胺基酸序列所作樹狀親源分析,顯示 A群菌株 Xvt122其與 X. campestris pv. vesicatoria 85-10的親源關係較之 B群菌株 Xvt45為近。雖然 Xcv Xvt45存在有 2套 xopE2基因,一套座落在基因組上,另一套則位於質體上,剔除基因組上的 xopE2會降低病原菌的感染力。然而, Xcv X85-10和 Xvt122的 xopE2突變株並不會影響其病原性。此外,根據實驗結果顯示,無論是選殖自 Xcv Xvt122或 Xcv Xvt45的 XopE2皆能透過第三型分泌系統抑制由 HopPsyA所引發的過敏性反應,而異質表現 XopE2則會降低其在感病番茄品系的毒性,而這些生物性功能和 XopE2所具有的保留性三元催化胺基酸 (consensus catalytic triad)(159th cysteine) 與 His硫醇蛋白酶胺基酸 (thiol-protease His residue)(47th histidine) 無關。 |
英文摘要 | Pathogenicity of Xanthomonas campestris pv. vesicatoria (Xcv) causing bacterial spot disease on tomato (Lycopersicon spp.) and pepper (Capsicum spp.) requires the type III secretion system (T3SS) and T3SS effectors. In this study, we employed the AFLP technique to investigate the diversity of X. campestris pv. vesicatoria isolated in Taiwan, and consequently a XopE2 homologue was identified in all fourteen Xcv strains that have been classified into two groups. Phylogenic analysis of XopE2 amino acid sequences indicated that XopE2 of Xcv Xvt122 (group A) has a closer genetic distance to XopE2 of Xcv 85-10 than to that of Xcv Xvt45 (group B). Interestingly, although it was suggested that Xvt45 contains duplicated xopE2 genes, one being located on the genome and the other located on a large plasmid, a single copy deletion of xopE2 within the genome caused a substantial reduction in virulence, but no effect of xopE2 mutation on virulence of Xcv 85-10 and Xvt122 was observed. Furthermore, our results revealed that XopE2 of Xcv Xvt122 or Xcv Xvt45 was able to suppress HR in a T3SS-dependent manner and the heterologously-expressed XopE2 was sufficient to modulate the virulence on susceptible tomato plants. Their biological functions are not dependent on the consensus catalytic triad (159th cysteine) and thiol-protease His residue (47th histidine) of XopE2. |
本系統中英文摘要資訊取自各篇刊載內容。