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頁籤選單縮合
題 名 | Direct Evidence of the Symplastic Pathway in the Trap of the Bladderwort Utricularia gibba L.=絲葉狸藻捕蟲囊的共質體輸送 |
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作 者 | 莊淳喬; 莊迪喬; 劉景煌; | 書刊名 | Botanical Studies |
卷 期 | 52:1 2011.01[民100.01] |
頁 次 | 頁47-54 |
分類號 | 379.21 |
關鍵詞 | 絲葉狸藻; 螢光染劑; 共質體運輸; Carboxyfluorescein; Symplastic transport; Utricularia gibba; |
語 文 | 英文(English) |
中文摘要 | 水生開花食蟲植物絲葉狸藻 (Utricularia gibba) 是非常獨特的,它具備捕蟲囊捕捉水中小生物,及囊內具有吸收營養的四爪及二爪腺毛。目前有很多文獻探討狸藻的營養吸收途徑,例如有文獻報導利用放射性元素鑭追蹤以顯示其非共質體輸送途徑,但尚未有文獻報導其共質體運輸途徑。本研究先以食用色素為追蹤物質,在複式顯微鏡觀察絲葉狸藻捕蟲囊的吸收途徑,接著採用追蹤物質螢光染劑 (carboxyfluorescein diacetate, CFDA),以螢光顯微鏡及共軛焦雷射顯微鏡來觀察囊內 CFDA輸送途徑,以確認所觀察為共質體運輸途徑。CFDA本身具有細胞膜通透性,被囊內的酶分解成不可穿透膜的 carboxyfluorescein (CF),此為觀察韌皮部運輸的可靠螢光追蹤物質。由實驗結果中,我們認為囊內物質的輸送路徑如下:四爪腺毛尾端、基座、底層表皮、囊內表皮、囊柄、葉及莖。因為光合作用可以增加腺毛對色素的吸收速率,所以我們認為腺毛吸收物質是需要能量的反應。腺毛可以很快的吸收螢光染劑而食用色素則較慢,因此前者較適合觀察物質在腺毛的輸送路徑,後者較適合長久觀察。目前為止並沒有狸藻捕蟲囊內共質體輸送路徑的直接證據,而我們是首先以螢光染劑及食用色素,清楚地將囊內共質體輸送路徑完整的表現出來。 |
英文摘要 | To capture prey, Utricularia gibba, an aquatic angiosperm carnivorous plant, is equipped with specialized bladders containing bifid/quadrifid glands for nutrient absorption. Several studies have focused on the nutrient absorption and subsequent transportation in the bladderworts; more specifically, the apoplastic pathway has been demonstrated as employing radioactive uranium salts. Nevertheless, the symplastic pathway has not been unambiguously demonstrated. Herein, we initially used food dyes as tracers to monitor the absorption processes by light microscope. We then confirmed the observed symplastic pathway using another vital tracer carboxyfluorescein diacetate (CFDA). The absorption and transportation of the CFDA inside the traps were observed using epifluorescence microscope and confocal laser scanning microscope. Our data clearly suggested that the tracers were transported through different tissues in the following order: terminal cells, pedestal cells, basal cells, surrounding epidermal cells, nearby leaves, and, finally, stems. The process was found to be light-sensitive, suggesting that it is energy-dependent. The uptake of the fluorescent dye was observed within seconds, while that of food dyes required 2 to 3 h. Thus, CFDA provides better resolution, while the food dyes afford prolonged tracing procedures. Taken together, the findings lead us to conclude that the symplastic pathway is an important transportation process that has never been shown previously in Utricularia. |
本系統中英文摘要資訊取自各篇刊載內容。