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題 名 | 吸入奈米等級之二氧化鈦顆粒對於鼠肺功能影響的研究=Study the Effects of Inhalation of Nano Particles of Titanium Dioxide on the Lung Function in Rats |
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作 者 | 楊玉純; 蘇千玲; 陳冠豪; 陳宗鵠; | 書刊名 | 呼吸治療雜誌 |
卷 期 | 10:1 2011.01[民100.01] |
頁 次 | 頁1-12 |
分類號 | 415.4 |
關鍵詞 | 奈米顆粒; 二氧化鈦; 肺功能; 肺損傷; Nano particles; Titanium dioxide; Pulmonary functions; Lung injury; |
語 文 | 中文(Chinese) |
中文摘要 | 環境中奈米微粒的暴露所涉及的健康風險相當廣泛。不同的暴露途徑與不同生物體內的累積,都會造成不同程度的影響。奈米微粒由於表面效應與小尺寸效應,會使奈米物質產生物理上或化學上性質的改變,進而對生物體產生危害。在日常生活中舉凡穿著、居住,奈米也被廣泛應用。另外,奈米材料中二氧化鈦奈米顆粒所具有之光觸媒及抗污自潔功效亦被廣泛應用於建築空間中。然而近年來過度強調奈米神奇功能,而忽略了奈米是否具有反向的效果,因此希望透過此研究以了解奈米顆粒對於呼吸道和肺部的影響。 在此研究中透過乾粉氣體噴射器將三種劑量的二氧化鈦奈米顆粒噴射到在氣體麻醉狀態下的大白鼠的肺內,實驗共分爲四組,分別爲對照組、低劑量4mg/kg暴露組、中劑量10mg/kg暴露組及高劑量20mg/kg暴露組,暴露八小時,在暴露期間觀察並記錄動物行爲及體重變化。肺功能的測量包含了非侵入型及侵入型肺功能測量儀器。在暴露前後使用BUXCO公司所生產之非侵入型鼠肺功能監測系統監測呼吸道阻力及吐氣尖峰流速。暴露八小時後利用BUXCO公司所生產的動物專用肺功能測量儀器測量功能性肺餘容積及肺部順應性。血液樣本中反應細胞損傷指標之一的乳酸脫氫酶之濃度變化也一併分析。 實驗結果顯示在二氧化鈦奈米顆粒暴露後,呼吸道阻力明顯增加及吐氣尖峰流速明顯減少。功能性肺餘容積有增加趨勢及肺部順應性上升。血液中乳酸脫氫酶濃度增加。透過此研究指出了奈米二氧化鈦顆粒暴露可引起呼吸道發炎反應、肺部過度充氣及肺部損傷。不同劑量的二氧化鈦暴露並未產生明顯的差異,說明了即使在低劑量的二氧化鈦的暴露也會引發呼吸道炎症反應以及肺部的傷害。 |
英文摘要 | Background and objective: Environmental nano particles exposure is a risk factor for health, in which different exposure routes or different amount accumulated produce different influences on the individuals. Since the surface effect and mass effect of nano particles they will change the physical or chemical properties of the bio-materials so that organisims will be injuried. Nano particles used widely in the daily life. For architecture, nano particles used in the coating and photocatalyst painting with anti-bacteria effect. Amazing functions of nano particles are over emphasized in recent decade. However, the reverse effects were ignored. Therefore, the effects of nano particles on lung function will be studied. Methods: In this study dry powder injector is used for titanium dioxide nano particles exposure after Sprague Dawley rats were anesthetized with Isoflurane. Different groups are included: control group, low doses of titanium dioxide nano particles (4 mg/kg), medium dose (10 mg/kg) and high dose (20 mg/kg) group. The duration of exposures is eight hours. During exposure period, body weight, behavior and appearance of SD rats are recorded. The pulmonary function tests including Non Invasive Airway Mechanics and BUXCO Pulmonary Function Testing. The parameters of lung injury included airway resistance, peak flow, functional residual capacity and lung compliance. Blood concentrations of lactate dehydrogenase were also measured. Results: Preliminary result showed that exposure to titanium dioxide nano particles, the respiratory resistance, functional residual capacity and compliance increased significantly. Peak flow decreased significantly. Lactate dehydrogenase level increased. Conclusions: Through this study, exposure of titanium dioxide nano particles can induce airway inflammation, lung hyperinflation and lung injury. There were not significant differences among different exposure doses suggests that even low dose of titanium exposure could induce lung inflammation and lung injury. |
本系統中英文摘要資訊取自各篇刊載內容。