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頁籤選單縮合
| 題 名 | 臺灣不同氣候區苦楝族群之遺傳多樣性=Genetic Diversity among Melia Azedarach L. Populations at Different Climatic Regions in Taiwan |
|---|---|
| 作 者 | 何坤益; 柯乃瑛; | 書刊名 | 作物、環境與生物資訊 |
| 卷 期 | 7:3 2010.09[民99.09] |
| 頁 次 | 頁195-211 |
| 分類號 | 377.2231 |
| 關鍵詞 | 苦楝; 氣候區; 簡單序列重複區間; 遺傳多樣性; Melia azedarach L.; Climate zone; ISSR; Genetic diversity; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 摘要 苦楝(Melia azedarach Linn.)屬於楝科(Meliaceae)固有速生樹種,廣泛分布臺灣,本研究採 集6 處氣候區之18 地區計130 個樣木,應用簡單序列重複區間(Inter-Simple Sequence Repeat; ISSR)分子指紋標誌,探討族群遺傳多樣性。使用8 組ISSR 引子進行PCR 擴增試驗獲得多型 性條帶44 條(66.67%)。族群遺傳分析(POPGENE)結果,顯示偏高基因歧異度、明顯遺傳分化 及族群基因不流暢(Nei’s 基因歧異度(H)為0.3930,遺傳分化(Gst)為0.4153,基因流(Nm)為 0.7040)。分子變方分析(AMOVA)結果,發現氣候區間變方成分為19.30 % (p<0.001),氣候區 內族群間變方成分為14.03% (p < 0.001),氣候區內之族群內個體間變方成分為66.67% (p < 0.001),個體間的遺傳變異為族群總變異的主要來源。各氣候區之族群間已有分化趨勢,其氣 候區之影響已達19.30%。關聯分析(Mantel test)顯示族群遺傳變異與氣溫、日照時間與地理距 離有顯著關係,表現各地區族群的遺傳變異受氣候區之影響。綜言之,氣候區之族群繁殖與散 佈受到氣候環境之影響,而表現族群遺傳多樣性。 |
| 英文摘要 | ABSTRACT Melia azedarach Linn., a member of Meliaceae, is a fast-growing native tree widely distributed in Taiwan. The present research used the technology of Inter-Simple Sequence Repeat (ISSR) molecular fingerprint to study the chemical composition and genetic diversity of this species. One hundred and thirty samples of M. azedarach. were collected from eighteen areas in six climatic regions of Taiwan. Eight primers were used and forty-four polymorphic sites were obtained. Population genetic (POPGENE) analysis revealed that total gene diversity (H) was 0.3930 and genetic index (Gst) was 0.4153, while gene flow index (Nm) was 0.7040. The analysis of molecular variance (AMOVA) showed that the variance component between population regions was 19.30% (p < 0.001), among populations within region was 14.03% (p < 0.001), and among individuals within population was 66.67% (p < 0.001). The results also indicated that the major variation existed among individuals within populations. However, a significant genetic differentiation existed among regions and among populations within regions. By exploring the habitat of the genetic differentiation among populations and gene flow between different climate regions, climate was found accounted for nearly 20% of variation. From the mantel test, results showed that there were significant differences in genetic variation with average temperature, average annual sunshine hours and geographical distance. In conclusion, the gene flow among populations was relatively restricted. Climate segmentation may be a significant factor to the genetic differentiation among these populations. |
本系統中英文摘要資訊取自各篇刊載內容。