查詢結果分析
來源資料
頁籤選單縮合
題 名 | 補益類中藥保護幹細胞及影響癌病患腫瘤細胞之免疫相關之功能基因體研究(2-2)=A Functional Genomic Study of Some Bu- Yi Chinese Herbs' Pharmacologic Effects on Protection of Tissue Stem/Progenitor Cells and Immuno-modulation of Patient's Cancer Cells (2-2) |
---|---|
作 者 | 施子弼; | 書刊名 | 中醫藥年報 |
卷 期 | 28:7 2010.09[民99.09] |
頁 次 | 頁1-32 |
分類號 | 414.5 |
關鍵詞 | 幹細胞; 前列腺癌細胞; 補益類中藥; 功能性; Human tissue stem/progenitor cell; Prostate cancer cell; Bu-Chi Chinese medicine; Functional genomics; |
語 文 | 中文(Chinese) |
中文摘要 | 幹原細胞具有多機能特性,是個體組織器官發育之起源,特別是當個體 損傷如同癌症之化療、放射線治療等面臨環境壓力下,幹細胞對於組織傷害 復原顯的格外重要。 本研究計畫延續探討補益類防護人體幹細胞分子藥理,同時亦將平行比 對分析補益類中藥對癌組織細胞之影響。本年計畫針對男性前列腺癌,深入 探討四物湯(4D)及六味丸(6D)等保護人體幹細胞分子藥理異同,以對癌組織 細胞之不同反應尋找中藥輔助癌症醫療應用契機。 具體實施方法將利用RT-PCR及定量PCR陣列等技術,探討補益中藥對 癌細胞及正常體組織幹細胞在放射照射(RI)壓力下影響致癌基因、腫瘤抑制 基因、細胞凋亡基因、細胞週期基因、前驅細胞早期基因及生長分化訊息傳 導基因之表達特性變化。利用螢光免疫染色分析前列腺癌腫瘤細胞附著分 子,以及表面蛋白標記受放射線壓力及四物湯藥效之影響。同時也利用細胞激素蛋白微陣列分析探討四物湯影響前發炎因子、細胞激素、趨化激素、介白素及其他細胞生長分化因子等蛋白表達之分析。實驗結果顯示補益中對四 物湯在RI(放射線)傷害壓力下對不同之前列癌細胞有不同之反應及效益。 其在RI(放射線)傷害壓力下對PCC2前列癌細胞有明顯的失去修復之加層效 益。但似乎對 Androgenic independent之前列癌細胞負面作用最為明確,顯示 四物湯抑制癌細胞之凋亡,促進週期基因Cyclind D等基因表達對癌細胞輔助 醫療之負面多於正面效益。在RI(放射線)傷害壓力下,六味丸對前列腺癌 腫瘤病患細胞之初步探討亦有增加腫瘤基因PSCA及癌細胞之表面附著相關因 子CD44表型之表現等多項負面作用,但在沒RI傷害壓力情況下反之對PCC3 前列癌細胞有顯著的存活成長抑制效用與對正常幹細胞及未確定癌之前列腺 腫病患之保護效益有顯著差異,顯示六味丸之服用效用在於保護正常細胞、防護癌之形成作用但不適用於放射醫療癌症效益,以及病症不同用藥之效果 具大差異。兩者均顯示補益中藥在防癌及癒後修復之個人醫療應用之機能差 異。初步結果有賴於進一步的分子微陣列分析確認。 |
英文摘要 | Aim: In this proposal study, we aimed to compare the pharmagenomic effect of Bu-Yi prescriptions on gamma-ray irradiated human prostate cancer cells and normal MSCs/fibroblasts by subtraction analyses of molecular profile changes between them. And hope to find out the drug candidate that may uniquely protect or help the stress release of normal tissue stem/progenitor cell but not the malignant tumor cells. Method: The drug effect on prostate cancer cells and mesenchymal stem/progenitor cells will be qualitatively and quantitatively evaluated by cytokine protein array, immunoflouence stain, and quantitative PCR super array under the physical and chemical stressed culture conditions. Specifically, we will also focus on analyzing the expressions of oncogenes, tumor suppressor genes, apoptotic genes, early genes, cell adhesion/interactive molecules, proinflammatory factors, cytokines and growth factors. Systemic integration with information from results of protein array and quantitative PCR super array will be analyzed subsequently. Results and Discussion: We have analyzed the Bu-Yi Chinese Drug's pharmacologic effect on protection and repair of tissue stem/progenitor during cancer therapy in functional genomic and proteomic level. Results obtained from this study showing that Su-Wu Tang (四物湯) enhances cell cycle gene Cyclin D expression, and inhibits cell apoptotic genes indicating the negative effect may influent the cancer irradiation therapy. The result also showed that Su-Wu Tang (四物湯) exhibits heterogeneous responses to different prostate cancer pataient cells, prostate cancer cell lines and normal MSCs.This property is also shown in the Lio-Wei Wan (六味丸) which exhibits enhance PSCA gene expression and CD44 cancer cell adhesion molecule of prostate cancer clls or cell lines, but on the other hand presented more preventive and protective effects to the non-cancer detected prostate patient and normal stem cells.These informations will be organized and served as a pharmacogenomic evaluation reference for the application of Chinese medicine on promoting normal stem cell and discuraging cancer cell in patient's body. |
本系統中英文摘要資訊取自各篇刊載內容。