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相關文獻
- 冬蟲夏草菌絲體對A群鏈球菌感染的保護效果之研究
- 臺灣地區之風濕熱與風濕性心臟病
- 嚴重A群鏈球菌感染
- Characteristics of Group A Streptococcal Bacteremia with Comparison between Children and Adults
- 侵襲性A群鏈球菌感染之致病機轉與治療趨勢
- Group a Streptococcal Septicemia with Retropharyngeal Abscess: A Case Report
- 臺灣地區A群鏈球菌之血清型及抗藥性的研究
- 仍具危險性的疾病--兒童A群鏈球菌感染
- A群鏈球菌引起的嚴重感染
- 侵襲性A群鏈球菌感染
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題名 | 冬蟲夏草菌絲體對A群鏈球菌感染的保護效果之研究=Protective Effect of Cordyceps Sinensis Mycelium in Group A Streptococcal Infection |
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作者 | 莊偉哲; | 書刊名 | 中醫藥年報 |
卷期 | 28:6 2010.09[民99.09] |
頁次 | 頁131-152 |
分類號 | 414.5 |
關鍵詞 | 冬蟲夏草菌絲體; A群鏈球菌; 感染小鼠模式; Cordyceps sinensis mycelium; Group A streptococcus; Infection mouse model; |
語文 | 中文(Chinese) |
中文摘要 | 研究目的: A群鏈球菌的感染會造成多種病症,包括輕微的咽喉炎、膿皰病、猩紅 熱,到致命的壞死性筋膜炎及毒性休克症候群,也可能造成非化膿性後遺 症,如急性腎絲球腎炎和風濕性心臟病。儘管現今有一些有效的抗生素,但 近年來,由A群鏈球菌感染造成嚴重疾病在全世界都有增加的趨勢。在台灣 各個年齡層的病人都可能引起壞死性筋膜炎、毒性休克症候群、及非化膿性 後遺症,而且目前仍無疫苗可用。先前在小鼠的研究顯示,冬蟲夏草菌絲體 對A群鏈球菌的感染提供保護效果。我們並且發現冬蟲夏草菌絲體會誘發細 胞激素的產生,而使得A群鏈球菌產生的毒素streptococcal pyrogenic exotoxin B (SPE B)所造成的吞噬作用的抑制有回升的現象。 研究方法: 為了分析冬蟲夏草菌絲體在細菌感染的功效,利用先前建立的A群鏈球 菌從氣囊感染小鼠的模式,並且以餵食方式給予冬蟲夏草菌絲體萃取物。計 畫採用large-scale screening,以cDNA microarray以及protein array大量篩選與發 炎反應、免疫反應及訊息傳遞相關基因表現的變化。同時,將冬蟲夏草菌絲 體萃取物分成不同fractions,以A群鏈球菌感染小鼠模式,探討不同fractions 可能提供的保護效果,比較小鼠存活率,並偵測局部組織和器官傷害程度、 細菌生長與散佈、以及chemokines和cytokines的產生。 結果與討論: Microarray、protein array以及RT-PCR的結果顯示,與發炎反應相關的 cytokine及chemokine明顯受到調節。如IL1鮻、IL3、IL3Rb、IL-10、IL12、 TNF-鯂、sTNF-RII、CTACK、CXCL16、Eotaxin1、MIG、MIP-1、TCA-3, 在餵食冬蟲夏草菌絲體萃取物的小鼠在受到A群鏈球菌感染的組別中,其表現量皆高於正常老鼠、單獨餵食冬蟲夏草菌絲體萃取物,或單獨感染A群鏈 球菌的小鼠。進一步以不同的fraction餵食小鼠,結果顯示餵食冬蟲夏草菌絲 體萃取物的小鼠,皆有延長的存活率,其中又以餵食Fraction 2的效果最好。 不論是蟲草菌絲體粗萃取物、Fraction 1或是Fraction 2,皆能有效地抑制感染 A群鏈球菌小鼠背部組織的滲血情形,A群鏈球菌感染小鼠造成菌血症的情 形,有餵食蟲草菌絲體粗萃取物、Fraction 1與Fraction 2的小鼠,其菌血症的 發生率也比單獨感染A群鏈球菌的小鼠組別低,其中以餵食蟲草菌絲體粗萃 取物與Fraction 2的小鼠效果最為明顯。另外,在in vitro的研究結果發現蟲草 菌絲體粗萃取物、Fraction 1與Fraction 2都可以活化單核細胞U937,增加細 胞的吞噬作用與毒殺細菌的能力。因此,蟲草菌絲體粗萃取物、Fraction 1與 Fraction 2可以增加對A群鏈球菌的感染提供保護效果。 |
英文摘要 | Aim: Group A streptococcus (GAS) causes a wide spectrum of diseases, ranging from mild pharyngitis, impetigo, and scarlet fever to life-threatening diseases including necrotizing fasciitis and streptococcal toxic shock syndrome. Nonsuppurative sequelae, such as acute glomerulonephritis and rheumatic heart disease, may also occur. Despite the availability of effective antimicrobial agents, there has been a worldwide increase in the incidence of serious invasive GAS infection in recent years. In Taiwan, patients of all ages with necrotizing fasciitis, toxic shock syndrome, and post-infection sequelae have been reported. No vaccine is available. Our previous studies have shown that Cordyceps sinensis mycelium (CS) protected mice from GAS infection. We also demonstrated that CS abrogated the streptococcal pyrogenic exotoxin B (SPE B)-mediated suppression of phagocytosis in monocytic cells by inducing the production of cytokines. Method: To further characterize the effects of CS in microbial infections, mice were inoculated with GAS via an air pouch with or without force-feeding with CS. We used large-scale screening by cDNA microarray and protein array to examine the inflammation-, immune response-, and signal transduction-related gene expression in mouse spleen cells after GAS infection with or without CS treatment. We further fractionated and investigated the protective efficacy of different fractions using the mouse model of GAS infection. Mouse survival rates, local tissue and organ damage, bacterial growth and dissemination, and chemokine and cytokine production were compared between mice given different fractions of CS extract. Results and Discussion: Microarray, protein array and RT-PCR analysis showed the increased expression of cytokines and chemokines, including IL IL3, IL3Rb, IL-10, IL12, TNF-鯂, sTNF-RII, CTACK, CXCL16, Eotaxin1, MIG, MIP-1, and TCA-3 in mice after GAS infection with CS treatment. Animal model was used to test whether the CS extract or fractions would provide the protection against GAS infection. Mouse survival rates were increased in CS extract and fractions pretreated groups. Among them, Fraction 2 conferred the best protective effect. CS extract and Fractions 1 and 2 all dramatically reduced local skin tissue damage. Bacterial dissemination rates were lower in CS-pretreated and Fractions 1 and 2-pretreated mice as compared with in untreated mice at 48 h after bacterial inoculation. Among them, CS- and Fraction 2-pretreated mice showed the most effective protection. The in vitro studies indicated that CS extract and fractions could activate monocytic cell line U937 to enhance phagocytic activity and bactericidal ability. Therefore, CS extract and fractions provide protection in the host against GAS infection. |
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