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| 題 名 | 鹽分對番茄根部有機酸及蛋白質表現之影響=Effect of Salt on the Organic Acids and Proteins of Tomato Root |
|---|---|
| 作 者 | 林永鴻; | 書刊名 | 高雄區農業改良場研究彙報 |
| 卷 期 | 18:2 2007.12[民96.12] |
| 頁 次 | 頁22-33 |
| 分類號 | 4 435.272 |
| 關鍵詞 | 番茄; 耐鹽性; 生理; 有機酸; 蛋白質; Tomato; Salt tolerance; Physiology; Organic acids; Proteins; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 番茄為耐鹽性作物,然而其耐鹽生理至今尚未完全被確認,本研究乃探討番茄生長於不同氯化鈉 (NaCl) 濃度之環境中,有機酸及蛋白質的變化情形,做為改進高鹽分環境中作物栽培之參考。分別將番茄苗種植於含0, 0.25% 及0.5% NaCl之水耕環境中,二週後抽取根進行有機酸及蛋白質分析,以瞭解鹽分對根部有機酸及蛋白質的影響。結果顯示,在高鹽環境(0.5% NaCl)中,與含0%及0.25% NaCl處理之番茄的根比較,草酸、蘋果酸及延胡索酸明顯較高,且有10種蛋白質具明顯表現,9種蛋白質則受到抑制,在明顯表現的蛋白質中,P69C, Cytochrome P450 proteins, Lucinerich protein, phototropin-1及Retrotransposon gag protein對高鹽分逆境的抵禦能力及根生長的促進具正面效應,因此使番茄的根生長不會受到抑制,而ATP synthase beta subunit protein因促進根呼吸之TCA cycle過程中能量的形成,促使檸檬酸及琥珀酸加速轉化成蘋果酸、延胡索酸及草酸而使得這三種有機酸大量累積,進而將鈉離子排除於根外,至於在0.5%NaCl環境中受到抑制的蛋白之中,TNF receptor-associated factor(ISS)及pr1-like protein較不含NaCl及含0.25% NaCl處理之含量低,然而因其機制未明,值得進一步的探討。本試驗至少證實,番茄根生長在不同NaCl濃度的環境中,其根部產生的有機酸及蛋白質是不同的,至於這些有機酸及蛋白質的耐鹽機制則有待進一步的研究。 |
| 英文摘要 | Tomato is a salt-tolerant crop. However, its physiology on salt-tolerance has not been clearly understood. The purposes of this research are conducted to evaluate changes of organic acids and proteins in tomato grown in environment of different NaCl concentrations and to provide a reference for improving crops cultivated in salt polluted environment. Tomato was cultivated in hydroponic solution separately at 0, 0.25% and 0.5% NaCl for two weeks. Then the roots were extracted for analyses of organic acids and proteins in order to understand the salt effects on root secretion of organic acids and proteins in roots. Compared with 0 and 0.25% NaCl treatment, the roots in 0.5% NaCl apparently secreted more oxalic acid, malic acid and fumaric acid, and 10 proteins in which P69C, Cytochrome P450 proteins, Lucinerich protein, phototropin-1 and Retrotraneposon gag protein, showed upregulated effects on salt tolerance , so that root growth was not inhibited. However, there were some proteins were inhibited. ATP synthase beta subunit protein might provide root respiration in the TCA cycle, in which citric acid and succinic acid were quickly converted to malic acid, fumaric acid and oxalic acid resulting in their large accumulation. Among the proteins which was inhibited by 0.5% NaCl, TNF receptor-associated factor (ISS) and pr1-like protein were secreted less than they were in 0 and 0.25% NaCl solutions. This will require further investigation. This research confirms that tomato grown in different NaCl concentration environments, its root will produce different organic acids and proteins whose salt-tolerance mechanisms require further investigation. |
本系統中英文摘要資訊取自各篇刊載內容。