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| 題 名 | 甜瓜白粉病菌之繁殖、接種及保存=Propagation, Inoculation and Preservation of Podosphaera xanthii Caused Muskmelon Powdery Mildew |
|---|---|
| 作 者 | 黃晉興; 陳純葳; 蘇俊峰; | 書刊名 | 臺灣農業研究 |
| 卷 期 | 58:3 2009.09[民98.09] |
| 頁 次 | 頁176-184 |
| 分類號 | 435.26 |
| 關鍵詞 | 甜瓜; 白粉病菌; 繁殖; 接種; 保存; Melon; Podosphaera xanthii; Powdery mildew; Propagation; Inoculation; Preservation; |
| 語 文 | 中文(Chinese) |
| 中文摘要 | 以挑單一孢子的方式可將甜瓜白粉病純化培養於甜瓜葉圓片(直徑1.5 cm)上,於20-24℃培養14-16天可獲得大量可吹落之分生孢子(4.5×10^4-6.8×10^4 conidia/leaf disc),發芽率24.8-42.3%,而16℃培養16-19天可產生3.2×10^4-3.5×10^4 conidia/leaf disc 之分生孢子,發芽率22.5-33.5%,12 及28℃產孢量較少且發芽率較低,而8及32℃的環境下無法產孢。以直接吹落分生孢子方式較噴佈分生孢子懸浮液之接種方式為佳,分生孢子發芽率分別為42.0及23.3%,而以(10±1)conidia/平方公分的接種源密度接種於盒栽離葉之發病度分別為70.5及44.5%,均有顯著差異。在24及4℃的環境下,白粉病菌分生孢子分別僅能維持7或30天的活力,在-30℃或液態氮中(-196℃)的低溫下,未乾燥之分生孢子均無法存活,而乾燥後的分生孢子持續保存180天後,仍可再感染葉圓片。 |
| 英文摘要 | Single conidium of Podosphaera xanthii, causal agent of powdery mildew of muskmelon, were isolated from colonies on diseased leaves and cultured on the leaf disks of muskmelon for purification and production of conidia. The range of optimum temperature for production and germination of conidia of P. xanthii was 20 to 24℃. When conidia were inoculated on the leaf disks (1.5 cm in diam.) by the air-blow method and incubated at 20 or 24℃ for 14-16 days, the fungus produced 4.5×10^4-6.8×10^4 conidia/leaf disc with the germination rates of 24.8-42.3%. When the inoculated leaf disks were incubated at 16℃ for 16-19 days, the number of conidia produced (3.2×10^4-3.5×10^4 conidia /leaf disc) and the rate of conidia germination (22.5-33.5%) were highest at the temperature. The conidia number and germination rate were low for the cultures incubated at 12 or 28℃. The cultures failed to produce conidia on the leaf disks incubated at 8 or 32℃ for 14-16 days or longer. Inoculation of conidia of P. xanthii on leaf disks of muskmelon by the air-blow method was more effective than the spraying method using conidia suspensions. The germination rates and disease severity were 23.3% and 42.0%, respectively, for the inoculation method of conidia suspensions, compared with 44.5% and 70.5% for the inoculation of air-blow conidia. Fresh conidia remained viable for 7 days at 24℃ and 30 days at 4℃ but they were unable to survive by storing at -30℃ or in liquid nitrogen. However, air-dried conidia stored at -30℃ or in liquid nitrogen survived for at least 180 days. |
本系統中英文摘要資訊取自各篇刊載內容。