頁籤選單縮合
題名 | 利用葉片圓盤接種法篩選甜瓜抗白粉病品種=Development of Leaf-Disk Method for Screening Melon Varieties Resistant to Sphaerotheca fuliginea Race |
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作者 | 黃晉興; 王毓華; 羅朝村; | 書刊名 | 中華農業研究 |
卷期 | 51:4 2002.12[民91.12] |
頁次 | 頁49-56 |
分類號 | 435.261 |
關鍵詞 | 甜瓜; 白粉病; 葉片圓盤接種法; 抗病品種; Melon; Powdery mildew; Leaf disk; Resistant varieties; |
語文 | 中文(Chinese) |
中文摘要 | 本研究利用接種白粉病菌於甜瓜葉片圓盤上,來快速檢測甜瓜品系對白粉病菌之抗感病性。其檢測方法如下:取苗齡3或4星期無病原菌感染之甜瓜植株上半部之新葉,經1%次氯酸納溶液表面消毒2~3分鐘,無菌水漂洗三次後,以打孔器取下直徑1.5公分之葉片圓盤,正面朝上,置於盛有二層滅菌濾紙之容器中,添加適量之M-solution養液使濾紙完全濕潤並有游離水產生,以維持葉片圓盤之活力,以吹落分生孢子方式接種每平方公分50~100個孢子濃度之白粉病菌(Sphaerotheca fuliginea race 1),容器加蓋密封置於24℃/18℃(Day/night)光照12小時之定溫箱中,培養10天後記錄發病指數。在室內採用葉片圓盤法測試不同甜瓜品種對白粉病之抗感病性,並對照生長箱中幼苗接種及及田間植株自然感染之結果,經分析顯示其兩者之相關性高(相關係數分別為r²=0.9709及r²=0.7924,p=0.05)。因此葉片圓盤接種法可應用於測定甜瓜品系對白粉病菌之抗感病性。 |
英文摘要 | A leaf-disk method was developed for disease assessment of melon powdery mildew caused by Sphaerotheca fuliginea race 1. Melon seedlings grown in growth chamber for 4 to 5 weeks were used to prepare leaf disks. The new spread leaves of melon seedling were surface-sterilized by dipping in 1% sodium hypochlorite solution for 2-3 minutes, and rinsed in sterile distilled water three times. The surface-sterilized leaves were individually cut into 15-mm diameter leaf disks with a sterile cork border. The abaxial-up leaf disks were then placed in container with 2 pieces of sterilized filter papers. Suitable volume of M-solution was added to the filter papers to maintain vitality of the leaf disks. The leaf disks were inoculated with conidia (50~100 spores/cm²) of the pathogen, and then were incubated at 24℃ /18℃ (day/night) with a 12 hrs photoperiod for 10 days. For disease severity evaluation, results showed that there be a positive correlation among leaf-disk assay, seedling inoculation in growth chamber, and melon plants infected naturally in field. The relative coefficients (r²) between leaf-disks assay and seedling inoculation, and naturally infected plants were 0.9709 and 0.7924, respectively (p=0.05). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。