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題名 | 使用微隨體基因座偵測烏頭翁與白頭翁可能的雜交子代=Detecting Obscure Hybrids of Light-vented Bulbul (Pycnonotus sinensis formosae) and Styan’s Bulbul (P. taivanus) Using Microsatellite DNA |
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作者姓名(中文) | 許祐薰; 劉小如; 李壽先; 許育誠; | 書刊名 | 國家公園學報 |
卷期 | 20:1 2010.03[民99.03] |
頁次 | 頁26-36 |
分類號 | 388.015 |
關鍵詞 | 烏頭翁; 白頭翁; 雜交; 微隨體; Styan’s Bulbul; Light-vented Bulbul; Hybridization; Microsatellite; |
語文 | 中文(Chinese) |
中文摘要 | 本研究由目前已經發表的鳥類微隨體基因座中,篩選出適用於烏頭翁與白頭翁的遺傳標記,嘗試建立一套能區分烏、白頭翁的微隨體基因座,將之用於雜交個體的偵測,並試圖解析墾丁地區特有的烏頭亞型個體,是否為雜交所產生的後代。分析來自四種鳥、共67對的微隨體基因座引子後,找出其中九組基因座在烏、白頭翁或烏頭亞型個體中具有多型性,然族群分化程度FST值在烏頭翁和白頭翁間只有0.021。平均每基因座的對偶基因數為2.89-3.67個,雜合度的觀測值為0.236-0.433,期望值則為0.308-0.409。以其中符合哈地-溫伯格平衡的六個基因座進行貝氏分群測試,顯示所有分析的樣本應屬同一族群。對應分析的結果能解釋的變異量雖然有限,但烏頭翁、白頭翁和烏頭亞型三者在各軸的分佈上有顯著的差異,其中以烏頭翁和白頭翁的分化程度較明顯,烏頭亞型的遺傳組成則介於二者之間。意味外型近似烏頭翁的烏頭亞型個體,可能在其祖代中曾經有白頭翁的基因滲入。 |
英文摘要 | The main purpose of this study was to select a set of microsatellite markers in order to distinguish the Styan’s Bulbul, Light-vented Bulbul, and suspected hybrids (gray-faced Styan’s Bulbul). We screened 67 primer pairs developed from four bird species and found only nine of these loci were polymorphic in Styan’s Bulbul, Light-vented Bulbul and/or the gray-faced Styan’s Bulbul. After excluding two loci which were not in Hardy-Weinberg equilibrium, and one locus which was monomorphic in both Styan’s Bulbul and Light-vented Bulbul, only six polymorphic loci were used in subsequent analyses. The mean of alleles per locus varied from 2.89 to 3.67, mean of observed heterozygosity ranged from 0.236 to 0.433, and mean of expected heterozygosity ranged from 0.308 to 0.409. No significant population differentiation was found in Styan’s Bulbul, Light-vented Bulbul, or gray-faced Styan’s Bulbul, as revealed by low FST value and Bayesian population assignment test. However, correspondence analysis showed significant difference among the three populations on the first two axes (CA1 and CA2). This indicated that these markers could distinguish Styan’s Bulbul and Light-vented Bulbul. The gray-faced Styan’s Bulbul might be the offspring of backcross between hybrid bulbuls and Styan’s Bulbuls. More polymorphic microsatellite markers should be found to distinguish these two bulbuls and identify their hybrids. |
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