頁籤選單縮合
題名 | 檢測家禽流行性感冒病毒非結構蛋白質抗體的間接型ELISA=Detecting Antibody to Avian Influenza Virus NS Protein by Indirect ELISA |
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作者 | 邱怡昌; 陳怡彤; 葉修如; 王金和; Chiu, Yi-chung; Chen, Yi-tung; Yeh, Shiu-ru; Wang, Ching-ho; |
期刊 | 臺灣獸醫學雜誌 |
出版日期 | 20091200 |
卷期 | 35:4 2009.12[民98.12] |
頁次 | 頁316-320 |
分類號 | 437.245 |
語文 | chi |
關鍵詞 | 家禽流行性感冒; 酵素聯結免疫吸附法; 非結構蛋白質1; Avian influenza; Enzyme-linked-immunosorbent assay; Nonstructural protein 1; |
中文摘要 | 由於施打疫苗的雞隻其血清中不含有NS1抗體,而感染野外株的雞隻會產生NS1抗體,故發展檢測NS1抗體之間接型ELISA(NS-ELISA)以區別雞隻體內的抗體的來源。從A/chicken/Taiwan/2838V/00(H6N1)病毒中抽取RNA,增幅NS1基因,在原核細胞表現NS1蛋白質,利用親和層分析法純化蛋白質,塗鍍於微平板做為抗原,發展檢測抗NS1抗體的ELISA。為防止NS1蛋白質中殘留之蛋白質干擾NS-ELISA的效果,以低齡及高齡雞隻的陰性血清以檢測訂定判定標準。結果顯示NS-ELISA可區分出陽性與陰性血清,且不受殘留之蛋白質干擾,其敏感性及特異性分別為94%及97%。故可用於區別雞隻體內的抗體是由施打疫苗或是感染野外毒產生的。 |
英文摘要 | Due to no anti-NS1 antibody, an enzyme-linked immunosorbent assay (ELISA) using recombinant NS1 protein as an antigen (NS-ELISA) was developed to differentiate avian influenza virus (AIV)-infected from vaccinated chickens. The NS1-encoding gene of avian influenza A/chicken/Taiwan/2838V/00 (H6N1) virus was expressed in E. coli. The results showed that the sensitivity and specificity of this NS-ELISA were 94% and 97%, respectively. Therefore, this assay could be useful for serological diagnosis to distinguish poultry infected with influenza viruses from those immunized with inactivated vaccines. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。