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題 名 | 以阻斷型ELISA檢測抗家禽流行性感冒H5亞型抗體的臨床應用=Clinical Use of a Blocking ELISA for Detecting Anti-H5 Avian Influenza Antibody |
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作 者 | 朱文玉; 陳奕成; 王金和; | 書刊名 | 臺灣獸醫學雜誌 |
卷 期 | 34:4 2008.12[民97.12] |
頁 次 | 頁248-254 |
分類號 | 437.717 |
關鍵詞 | 家禽流行性感冒病毒; 酵素聯結免疫吸附法; H5亞型單株抗體; 血球凝集素; Avian influenza virus; Enzyme-linked-immunosorbent assay; H5-subtype monoclonal antibody; Hemagglutinin; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究之目的為評估全病毒阻斷型酵素聯結免疫吸附法(Virus-B-ELISA)之檢測效力,並簡化此Virus-B-ELISA以減短操作時間。方法為收集雞隻田間血清樣本,分別進行Virus-B-ELISA 及血球凝集抑制試驗(hemagglutination inhibition test, HI test),並以HI test之結果作為金標準,計算出Virus-B-ELISA 相對於HI test之敏感性及特異性。本研究亦針對此Virus-B-ELISA之病毒塗鍍步驟加以簡化,且使用已知H5抗體陰性或陽性之血清,分別進行原始Virus-B-ELISA及簡化之Virus-B-ELISA檢測,以比較二者間之差異。結果發現原始Virus-B-ELISA檢測雞隻血清之敏感性與特異性若與HI test相較分別為95.76% (113/118)和91.47% (268/293),步驟簡化前後Virus-B-ELISA之值為0.9539,顯示二種檢測之結果近吻合。以本實驗室製作之簡化阻斷型Virus-B-ELISA能更快速地偵測出H5亞型AIV抗體。 |
英文摘要 | The purposes of this study were to evaluate the efficiency of the blocking enzymelinked-immunosorbent assay by coating with the whole virus as the antigen (Virus-B-ELISA) and to modify the procedure to save time. The hemagglutination inhibition (HI) test was taken as the standard for calculating the sensitivity and specificity of the Virus-B-ELISA for detection of field chicken sera. This study simplified the virus coating step of the Virus-B-ELISA by drying and compared with that before drying. In original Virus-B-ELISA, the sensitivity and specificity based on the HI test was 95.76% (113/118) and 91.47% (268/293), respectively. The Kappa value between original Virus-B-ELISA and simplified Virus-B-ELISA was 0.9539, which reveals the latter assay provided high perfect consistency. Simplified Virus-B-ELISA provides way in the detection of AIV-H5 subtype antibody. |
本系統中英文摘要資訊取自各篇刊載內容。