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題 名 | 利用葉綠體matK基因序列分析原生石斛蘭之遺傳歧異度=Genetic Relationships of Dendrobium (Orchidaceae) Species Based on Chloroplast-matK Gene Sequences |
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作 者 | 李文立; 李碩朋; 陳甘澍; 林榮貴; 劉政道; 戴廷恩; 鍾仁彬; | 書刊名 | 臺灣農業研究 |
卷 期 | 58:1 2009.03[民98.03] |
頁 次 | 頁61-71 |
分類號 | 435.431 |
關鍵詞 | 石斛蘭; 葉綠體; 基因; 遺傳; 親緣分析; Dendrobium; Chloroplast; matK gene; Genetic relationships; Phylogenetic analysis; |
語 文 | 中文(Chinese) |
中文摘要 | 自不同原生石斛蘭基因組DNA中選殖葉綠體matK的基因片段經定序後比較其相似度,據以做爲不同原生石斛蘭之親緣分析與分類依據,以matK基因序列分析結果顯示:各石斛蘭間的相似度介於91.1至99.7%,顯示石斛蘭種間matK基因序列保守性高。細莖石斛(Dendrobium leptocladum)與其它參試石斛蘭之平均相似度爲91%,是差異性最大者;三星石斛 (Epigeneium sanseiense) 與連珠石斛(E. nakaharaei)二者間的相似度最高,達99.7%,經由matK 基因序列分析結果可將參試石斛蘭分爲4個群,其中第1群的遺傳距離爲0.003,第2群的遺傳距離則介於0.020–0.012之間;第3群的離散度較大,遺傳距離介於0.038-0.016之間,而細莖石斛(D. leptocladum)因爲DNA 缺失變異較多而與其他石斛蘭間差異性最大,遺傳距離介於0.089-0.074之間,故被單獨歸類成第4群,本種也是台灣原生石斛特有種。 |
英文摘要 | Dendrobium is an important genus of Orchidaceae, especially in Asia. In this study, chloroplast-matK sequences were used to identify genetic relationships of Dendrobium species. Results showed a high degree of similarity (91.1 to 99.7%) among different Dendrobium species tested, indicating that the matK gene sequences are highly conservative in Dendrobium. Similarity in D. leptocladum is 91.0% which is the most different from others Dendrobium species examined. Epigeneium san and E. nak had the highest similarity of 99.7% and, they were separated from the genus Dendrobium. Based on aligned sequences of matK regions, the 14 Dendrobium species were divided into four main groups with the genetic distance of 0.003 for group 1; 0.020-0.012 for group 2; 0.038-0.016 for group 3 and 0.089-0.074 in D. leptocladum for group 4. D. leptocladum is a group high genetic divergence due to mass deletion of its matK sequences through mutation and it is a native species in Taiwan. |
本系統中英文摘要資訊取自各篇刊載內容。