|The main purpose of this study is to know the effect of environmental education based on ecotourism to middle high school students' environmental awareness, attitude and behavior. The study sites are "Endemic Species Research Institute" in Ji-ji, and. "Tao-Mi Eco-Village" in Pu-li Nantou County. The surveyed samples are the students of Taiwan Adventist Academy located on Yuchi village in Nantou County. The students were asked to do sentence creative worksheets by using the words, "environment" and "ecosystem". Furthermore, the students also were requested to fill worksheet after tour was finished. The observation and interview in the activity also are carried it on by investigator. The main results are as followings: 1. Although the students cannot really know that the main belief of ecotourism including "environment", "visitors", and "residents" they can accept to pay for visiting at least. Moreover, they also understand the importance of environment protection. 2. The ecotourism can evoke students to have deeper and wider environmental awareness, change environment attitude toward to realize the importance of environmental protection, and result in asking themselves to express environment protective behavior. 3. The main promotions and constraints of the change of the environmental education content, the promotion factors include the individual sensational experiences, switching situation and obtaining rewards, the influence of environment, obtaining the knowledge from personal achievement, the positive factor of interpreter, and the enjoyment that gets along with classmates. The constraints include personal factor, the negative factor of interpreter, non-appropriate time arrangement, and other non human-control factor.