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題 名 | 以cDNA基因體微陣列分析進行前導性研究探討葉酸缺乏啟動人類肝癌細胞株侵襲性相關之訊息傳遞因子基因表現的影響=A Pilot Study of a cDNA Microarray Analysis of Cancer Invasion-Associated Signaling Gene Expressions in Folate-Deficient Human Hepatoma Cells |
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作 者 | 林振豪; 許瑞芬; | 書刊名 | 臺灣營養學會雜誌 |
卷 期 | 33:4 2008.12[民97.12] |
頁 次 | 頁124-138 |
分類號 | 411.3 |
關鍵詞 | 葉酸缺乏; 微陣列分析; 侵襲; Hep3B細胞; Folate deficiency; Microarray analysis; Invasion; Hep3B cell; |
語 文 | 中文(Chinese) |
中文摘要 | 葉酸缺乏對肝癌細胞侵襲性的影響,目前仍不清楚。本研究選用葉酸缺乏九天Hep3B肝癌細胞株和葉酸充分之對照組,進行cDNA微陣列分析總體基因表現之差異性。以基因分群方法(Clustering)篩選與腫瘤侵襲相關之訊息傳遞基因表現群組,並與文獻報導中具轉移侵犯性之人類肝癌組織所表現基因群組進行比對分析。結果顯示,葉酸缺乏對Hep3B細胞株啟動訊息傳遞因子基因表現具有正向調控效應的基因,包括:三個配體基因(HPSE、PTTG1和COL1A1);七個受器與膜蛋白基因(RHOC、KAl1、PLEC1、RAB38、VAPA、COL1A1和HLA-DPB1);三個訊息傳遞分子基因與致癌基因(CTNNB1、ASB13和TPl1);五個轉錄因子基因(MXl1、MAX、MYC、SNRPF和HNRPAB);一個與分解細胞外基質的酵素MMP家族基因(MMP9);三個表皮間葉轉換系統相關基因(CTNNB1、HNRPAB和PTTG1);三個血管新生基因(KDR、ANGPT2和HPSE)。然而葉酸缺乏啟動這些基因群組的放大表達量相對於控制組,並未達顯著性(P>005)。將葉酸缺乏啟動與轉移相關之群組基因和文獻中具轉移侵犯性之人類肝癌組織或細胞所表現基因群組進行比對,發現有40.0%-100%之基因表現具有相同調控趨勢。Hep3B培養於葉酸缺乏九天,其移動和侵襲能力並未顯著高於對照組。因此,本前導性研究以cDNA微陣列分析篩選出葉酸缺乏肝癌細胞中與人類轉移性肝癌組織或細胞具有相同的訊息傳遞分子基因指印,然而這些分子指印在葉酸缺乏九天被放大表達的程度,未來則要以即時定量PCR分析加以確認。 |
英文摘要 | The effects of folate deficiency on the invasive capability of hepatocellular carcinoma (HCC) remain unknown. The aims of the study were to investigate cancer invasion-associated gene expression signatures in folate deficient-human HCC cells. A complementary (c) DNA microarray analysis was applied to analyze changes in global gene expression patterns in Hep3B cells cultured in folate-sufficient and folate-deficient medium for 9 days. Tumor invasion-associated genes involving signaling pathways were clustered and compared to those genes expressed in invasive HCC tissues. The results showed that a folate deficiency caused the upregulation of gene expressions in signaling ligand molecules (HPSE, PTTG1, and COL1A1 ), receptor and membrane protein signaling molecules (RHOC, KAI1, PLEC1, RAB38, VAPA, COL1A1, and HLA-DPB1 ), oncogene molecules (CTNNB1, ASB13, and TPl1), and transcription factors (MXI1, MAX, MYC, SNRPF, and HNRPAB). Epithelialmesenchymal transition-associated genes (CTNNB1, HNRPAB, PTTG1, and MMP9) and angiogenesis-associated genes (KDR, ANGPT2, and HPSE) were also upregulated in folate-deficient hep3B cells. Although multiples of increased gene expressions between folate-sufficient and -deficient Hep3B cells were not statistically significant, 40.0%~100% of those invasion-associated gene categories were expressed in folate-deficient Hep3B cells, resembling the expression pattern found in invasive human HCC tissues and tumors previously reported in the literature. Consistently, the invasive and migratory capabilities of Hep3B cells did not change upon 9-day culture in folate-deficient medium. Taken together, this pilot study identified cancer invasion-associated signaling gene expressions in hep3B cells cultured for 9 days in folate-deficient medium. Those folate- and invasion-associated expressed gene signatures found by this cDNA microarray analysis warrant further confirmation using a real-time PCR method. |
本系統中英文摘要資訊取自各篇刊載內容。