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題 名 | Coordinated Expression of Sulfate Transporters and Its Relation with Sulfur Metabolites in Brassica napus Exposed to Cadmium=鎘脅迫油菜中硫轉運體的協同表達及其與硫代謝物合成之間的關係 |
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作 者 | 孫雪梅; 呂波; 黃思齊; 徐朗萊; 楊志敏; | 書刊名 | Botanical Studies |
卷 期 | 48:1 2007.01[民96.01] |
頁 次 | 頁43-54 |
分類號 | 435.24 |
關鍵詞 | 油菜; 硫轉運蛋白; 轉錄; Cd; Brassica napus; Cadmium; Sulfate transporter; Transcriptional Expression; |
語 文 | 英文(English) |
中文摘要 | 本研究從油菜植株中克隆到一個編碼低親和轉運蛋白基因全長cDNA(BnSultr2;2, accession number Q091257)。氨基酸序列比對發現該基因編碼的蛋白與擬南芥和印度芥菜中已知的低親和硫轉運蛋白具有很高的相似性,並具有12個跨膜結構域。轉錄表達顯示,缺硫處理能誘導油菜植株葉片BnSultr2;2基因轉錄水準的提高,而Cd(20-120μM)處理也能使葉片中BnSultr2;2轉錄表達呈上升趨勢,但根中的表達則明顯下降。為了探明硫轉運蛋白基因的轉錄表達活性與硫酸鹽的吸收和同化及其與Cd耐性之間的關係,我們還從根中分離到另一個已知高親和硫轉運蛋白基因BnSultr1;1(accession number AJ416460)。研究表明,該基因僅在根中表達,而且其表達水準在缺硫和Cd脅迫時均表現為上調。另外,當處理植株Cd的濃度為40μM時,根中的SO2-4、S-2、GSH、non-protein thiols含量均上升。上述結果表明油菜植物可能通過調節硫轉運蛋白的表達來促進油菜植株對硫的吸收和同化,從而達到緩解Cd毒害的目的。 |
英文摘要 | Full-length cDNAs encoding a putative low-affinity sulfate transporter (LAST), designated BnSultr2; 2 (accession number DQ091257) were isolated from Brassica napus 72 h after sulfate deficiency. BnSultr2; 2 shows significant similarity at an amino acid level with the available LASTs from the dicotyledonous Arabidopsis and Brassica Juncea. It displays the typical twelve-membrane spanning domains of other plant species’ sulfate transporters. Examination of steady-state mRNA levels revelas that BnSltr 2;2transcripts were enhanced in leaves of sulfate-deficient plants. The leat Bnsultr2;2 expression was also up-regulated under 20-120μM Cd exposure, but under the same conditions, the BnSultr2;2 expression in roots was severely suppressed. To understand the relationship between sulfate uptake and Cd stress, we simultaneously isolated another cDNA encoding a known high-affinity sulfate transporter (BnSultr1;1, accession number AJ416460) from roots. RT-PCR analysis demonstrates that BnSultr1;1 was expressed only in roots, and its expression was upregulated by both sulfate-deficiency and Cd exposure. To link up the expression of sulfate transporters to sulfur accumulation and assimilation, the concentrations of sulfate, sulfide, glutathione, and thiol-containing compounds in plant tissues were measured. Elevating Cd concentrations in the culture medium up to 40μM increased the accumulation of sulfate, sulfide, glutathione, and non-protein thiols in roots. These results suggest that a coordinated regulation of sulfate transporters enables plants to tolerate Cd stress via a efficient sulfate uptake and assimilation. |
本系統中英文摘要資訊取自各篇刊載內容。