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題 名 | Cloning and Sequence Analysis of Oilseed Rape (Brassica Napus) SHP2 Gene=甘藍型油菜中與角果開裂有關的基因 SHP2 的選殖及分析 |
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作 者 | 譚小力; 夏宗偉; 張麗麗; 張志燕; 郭忠建; 戚存扣; | 書刊名 | Botanical Studies |
卷 期 | 50:4 2009.10[民98.10] |
頁 次 | 頁403-412 |
分類號 | 435.242 |
關鍵詞 | 擬南芥; BnSHP2基因; 甘藍型油菜; 角果開裂; MADS-box 家族轉錄因數; 序列比對; Arabidopsis; BnaSHP2 gene; Brassica napus; Fruit dehiscence; MADS-box family transcriptional factors; Sequence alignment; |
語 文 | 英文(English) |
中文摘要 | 擬南芥中的SHATTERPROOF1 (SHP1) and SHATTERPROOF2 (SHP2) 屬MADS-box 家族轉錄因數,它們通過調控角果皮邊沿細胞的分化,來控制角果的開裂。用擬南芥SHP2 的序列在油菜的序列資料庫中比對,得到一批序列相似性較高的EST ,拼接成一個含有開放閱讀框的cDNA ,並在甘藍型油菜中得到選殖,命名為BnaSHP2 ,但其5' 和3' 的非翻譯區序列不全。通過RACE ,得到了兩個轉錄序列,分別命名為BnaA.SHP2.a 和BnaC.SHP2.b 。本研究對其中一條序列BnaA.SHP2.a 做進一步分析。BnaSHP2 蛋白和其他MADS-box 轉錄因數序列比對和系統分析表明,油菜BnaSHP2 和擬南芥AtSHP2 序列具有很高的相似性。Southern 雜交結果顯示BnaSHP2 基因在油菜基因組中至少存在兩個同源基因;半定量反轉錄PCR 結果說明BnaSHP2 主要在根、花蕾和角果中表達,而在莖、葉和種子中不表達。把BnaSHP2 和綠色螢光蛋白融合(EGFP),通過杆狀病毒系統,在棉鈴蟲細胞中表達,發現 BnaSHP2 只在細胞核中表達,證實了它作為轉錄因數的核定位特性。 |
英文摘要 | MADS-box family transcriptional factors SHATTERPROOF1 (SHP1) and SHATTERPROOF2 (SHP2) are required for fruit dehiscence by regulating the cell specification in valve margin in Arabidopsis thaliana. BLASTN search against Brassica database in TAIR using AtSHP2 gene as a query, revealed several ESTs sharing high similarity to AtSHP2. Two highly homologous cDNAs of SHP2 were obtained from Brassica napus by PCR and RACE, and they are named as BnaA.SHP2.a and BnaC.SHP2.b, respectively. In this work, we focused on BnaA.SHP2.a. Sequence alignment and phylogenetic analysis indicated that BnaA.SHP2.a. is highly identical to AtSHP2. The southern blotting demonstrated that there are at least two copies of BnaSHP2 in B. napus genome. Semi-quantitive RT-PCR showed that BnaSHP2 was expressed in roots, floral buds, and pods, but not in stems, leaves, or seeds. BnaSHP2 was localized in the nuclei of HzAM1 cells when fused with EGFP and expressed using HaSNPV bacmid system. |
本系統中英文摘要資訊取自各篇刊載內容。