查詢結果分析
相關文獻
- 紫錐菊組織培養苗體細胞變異之RAPD分析
- Detection of Somaclonal Variation in Tissue Culture Regenerants of Pangolagrass
- 水稻懸浮培養細胞再生植株體細胞變異在早期世代之表現
- 水稻組織培養再生植株農藝性狀之變異
- 應用增殖限制酵素DNA片段多型性分析於蝴蝶蘭花色性狀與變異鑑別的研究
- 臺灣新興藥用保健植物--紫錐菊簡介
- 利用組織培養大量繁殖雙鋸齒葉玄參
- 臺灣金線連之組織培養--種子成熟度及前處理對萌芽與幼苗發育之影響
- Calcium-Induced Changes on Crystallins in Organcultured Porcine Lens
- 植物組織培養瓶內微氣候之量測
頁籤選單縮合
題 名 | 紫錐菊組織培養苗體細胞變異之RAPD分析=RAPD Analysis of Somaclonal Variation of In Vitro Echinacea Plantlets |
---|---|
作 者 | 莊淑貞; 陳駿季; 周煒裕; 陳宗禮; 宋濟民; | 書刊名 | 作物、環境與生物資訊 |
卷 期 | 5:2 2008.06[民97.06] |
頁 次 | 頁85-92 |
分類號 | 435.435 |
關鍵詞 | 紫錐菊; 藥用保健植物; 組織培養; 體細胞變異; 逢機增幅多型性核酸; Echinacea purpurea; Medicinal plant; Micro-propagation; Somaclonal variation; RAPD; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究利用逢機增幅多型性核酸技術(random amplification of polymorphic DNA; RAPD)偵測紫錐菊Echinacea purpurea由葉片微體繁殖再生植株之體細胞變異,以10組RAPD逢機引子檢定6組包含母株的微體繁殖組共56個單株個體。結果顯示,10組RAPD逢機引子中4組表現多型性,其多型性條帶分別為UBC-44-1 kb、UBC-52-500 bp、UBC-58-500 bp及Operon-X3-750 bp。進一步利用條帶有無計算遺傳相似係數,其中由母株D3-9繁殖的10個再生植株中,有3個再生植株缺失這些條帶,這3個再生植株與母株的遺傳相似係數為0.926,由D3-9繁殖的其餘7個再生植株與母株的遺傳相似係數均為1。其餘由篩選母株T6-28、T3-23、T5-9、T2-15及D7-4繁殖衍生的40個再生植株與其母株的遺傳相似係數均為1.00,這5組微體繁殖組皆無體細胞變異。 |
英文摘要 | In the present study, random amplification of polymorphic DNA (RAPD) analysis was used to assess DNA variation among Echinacea purpurea plantlets regenerated from leaf organogenesis. Fifty-six regenerated plantlets, including donor plants, from six different groups were used for RAPD analysis using ten arbitrary primers. The results indicated that polymorphic profiles were detectable from 4 out of 10 primers, which were UBC-44-1 kb, UBC-52-500 bp, UBC-58-500 bp and Operon-X3-750 bp. The calculated coefficients of genetic similarity from those polymorphic profiles indicated that 3 out of 10 regenerated plantlets from donor plant D3-9 showed somalclonal variations. The similarity coefficients for these 3 plantlets and other 7 plantlets were 0.926 and 1.00, respectively. The 40 plantlets from donor plants T6-28, T3-23, T5-9, T2-15 and D7-4 showed no detectable somaclonal variation with similarity coefficients of 1.00. |
本系統中英文摘要資訊取自各篇刊載內容。