查詢結果分析
來源資料
頁籤選單縮合
題 名 | 受逆境調控之水稻醛糖還原酶基因之表現=Expression Profiling of Stress-responsive Aldose Reductase Genes from Rice |
---|---|
作 者 | 林威至; 李彥妮; 羅正宗; 吳志文; 洪傳揚; 黃文理; | 書刊名 | 作物、環境與生物資訊 |
卷 期 | 5:3 2008.09[民97.09] |
頁 次 | 頁171-179 |
分類號 | 434.111 |
關鍵詞 | 水稻; 醛糖還原酶; 非生物性逆境; 生物資訊; Oryza sativa; Aldose reductase; Abiotic stress; Bioinformatics; |
語 文 | 中文(Chinese) |
中文摘要 | 摘要 醛糖還原酶(aldose reductase)普遍存在 動植物體內,被認為與細胞解毒作用、糖醇 生合成及逆境抗性有關。本研究利用生物資 訊分析,針對水稻基因體主要資料庫進行搜 尋比對,發現在水稻基因組中至少可比對出5 個aldose reductase 基因(OsARs),為一多基 因族,分別位於第1、第5 及第10 條染色體 上,可編碼出305 到375 個不等長度之胺基 酸序列,與已發表aldose reductase 基因序 列比對,可區分為三群。設計專一性引子, 選殖出5 個OsARs 基因,進一步利用懸浮培 養系統,發現5 個OsARs 基因中,OsAR5 基因表現明顯受ABA 與sorbitol 誘導,屬於 逆境誘導基因;相反地,OsAR1 與OsAR4 基因表現則受ABA、NaCl 及sorbitol 抑制, 屬於逆境抑制表現基因,而OsAR2 與OsAR3 基因則為持續表現型基因。OsARs 基因於水 稻幼苗系統之表現分析,結果則與懸浮培養 系統不完全一致,其中OsAR4 與OsAR5 明 顯呈現根部專一性與ABA 誘導表現,OsAR3 則主要在地上部表現。本文為首篇觀察有關 水稻OsARs 基因表現之試驗研究報告,未來 將進一步深入分析水稻植株發育過程與不同 部位中OsARs 基因之表現,並釐清OsAR 基 因之生理功能及與逆境耐受性之關係。 |
英文摘要 | ABSTRACT Aldose reductase is widely distributed in animal and plant tissues and has been considered related to function in cellular detoxification, sugar alcohol biosynthesis, and stress tolerance. In the present study, genome-wide database search indicating at least five different aldose reductase genes (OsARs) were located at chromosomes 1, 5 and 10 of rice genome, respectively. OsARs belong to a multigene family and the encoded proteins have various lengths, ranging from 305 to 375 amino acids. Results of phylogenetic analysis showed that these proteins may be classified intothree main clusters. Genes cloning and expression patterns under stress treatments were further performed with suspension culture system. Results indicated that OsAR5 mRNA level was significantly increased in suspension cells treated with ABA and sorbitol, whereas no significant changes were observed for OsAR2 and OsAR3 isogenes under ABA and sorbitol treatments. On the other hand, the mRNA levels of OsAR1 and OsAR4 were decreased by ABA, salt, and sorbitol treatments. Results suggest that OsARs genes might closely relate to stress response. To our knowledge, this is the first report that regards OsARs gene expression pattern under stresses in rice. Further studies are necessary to unravel the possible roles of OsARs genes on stress resistance and sugar metabolism. |
本系統中英文摘要資訊取自各篇刊載內容。