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題名 | Effect of Intrathecal N[92b2]-nitro-L-arginine Methyl Ester Administration on Fos Expression in the Spinal Dorsal Horn in Rats Following Sciatic Nerve Ligation=脊椎腔內預先應用L-NAME對坐骨神經結紮大鼠脊髓c-fos表達的影響 |
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作者姓名(中文) | 李平; 薛富善; 李成文; 劉鯤鵬; 劉毅; 許亞超; 楊泉湧; | 書刊名 | 麻醉學雜誌 |
卷期 | 45:2 2007.06[民96.06] |
頁次 | 頁65-72 |
分類號 | 416.5 |
關鍵詞 | 痛覺過敏; 坐骨神經; 一氧化氮; 脊髓; Proto-oncogene proteins c-fos; Hyperalgesia; Sciatic nerve; Nitric oxide; Spinal cord; |
語文 | 英文(English) |
中文摘要 | 背景:在目前的文獻中,關於一氧化氮(nitric oxide, NO)的致傷害性感受或抗傷害性感受作用仍然存在著激烈的爭論。作為中樞神經系統神經元啟動的標誌之一,Fos表達已被廣泛用於評價外周傳入信號所誘發的中樞神經活動變化。為了明確NO在誘發Fos表達方面的作用,我們設計該試驗研究觀察了脊椎腔內預先應用非特異性一氧化氮合(NOS)抑制劑NG-硝基-L-精氨酸甲酯(L-NAME)對坐骨神經結紮大鼠熱痛閾和脊髓後角c-fos基因表達的影響。方法:選擇脊椎腔內置管1周後無神經損傷症狀的SD大鼠80隻,在實施坐骨神經結紮(CCI)和假手術前15分鐘向脊椎腔內注射L-NAME 250μg (10μl)或等容量的生理鹽水。在術後第1、第3、第7和第14天,採用後爪退縮潛伏期(paw withdrawal latency, PWL)測量其熱痛閾,並採用免疫組化方法觀察其同側脊髓背角c-fos基因的表達情況。結果:與未做處理的動物相比,雖然CCI和假手術均能引起大鼠脊髓後角fos的迅疾和長期表達,但是只有CCI大鼠出現了後爪退縮潛伏期的顯著縮短。脊椎腔內預先應用L-NAME使CCI大鼠第3、第7和第14天同側脊髓背角Fos陽性神經元的數目分別降低了54%、57%和43%,並伴有熱痛覺過敏反應的顯著減輕。然而脊椎腔內預先應用L-NAME對假手術誘發的脊髓c-fos表達則無影響。結論:脊髓Fos表達可由不同的機制所誘發,並且不應將其視為指示疼痛疾病發生的可靠標誌。在周圍神經損傷介導的中樞敏感化方面,NO對傷害性感受的產生和脊髓Fos表達則發揮著重要作用。 |
英文摘要 | Background: In the available literature, the pro- or antinociceptive role of nitric oxide (NO) is warmly disputed. As a marker of neuronal activation of the central nervous system, Fos expression has been widely used to assess the change in central neuronal activity evoked by peripheral input. In this study, we examined the effect of intrathecal L-NAME, a non-selective nitric oxide synthase (NOS) inhibitor, on nociceptive behavior and spinal Fos expression in rats following chronic constriction injury (CCI) of sciatic nerve, a model of neuropathic pain similar to that observed in clinical setting. Methods: Eighty adult male SD rats showing no neurological deficiency a week after intrathecal catheterization were used in this study. L-NAME 250 g of 10 l (an equivalent or 0.9% saline) was injected intrathecally 15 min prior to CCI or sham operation. In addition to examination of thermal hyperalgesia by paw withdrawal latency (PWL), measurement of Fos protein staining neurons in the lumbar spinal cord using an immunohistochemistry technique were made at 1, 3, 7 and 14 days after operation. Results: As compared with untreated animals, both CCI and sham operations evoked an early and long-term Fos expression, whereas a significant decrease in PWL was demonstrated only in rats receiving CCI. On days 3, 7 and 14 after CCI, the number of FLI neurons in the spinal dorsal horn ipsilateral to the injury decreased by 54%, 57%and 43%, respectively, in CCI-L-NAME group when compared with CCI-saline group, corresponding to the significant attenuation of thermal hyperalgesia. However, intrathecal L-NAME preadministration had no effect on the spinal Fos expression evoked by sham operation. Conclusions: Spinal Fos expression could be induced by different mechanisms, and it should not regarded as a reliable marker of pain sensation disorders. NO plays an important role in the development of nociception and spinal Fos expression through central sensitization mediated by peripheral nerve injury. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。