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題 名 | Freezing Wheat Root Tips Causes Hyperphospharylation of Histone H3 at Serine 10 in the Cell during Mitosis=冷凍處理小麥根尖可以引起細胞中組蛋白H3 ser-10位點的超磷酸化現象 |
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作 者 | 宋麗萍; 李登文; 周浩; 劉如明; 曹扣; 陳家童; 黃熙泰; | 書刊名 | Botanical Studies |
卷 期 | 49:1 2008.01[民97.01] |
頁 次 | 頁19-24 |
分類號 | 434.124 |
關鍵詞 | 免疫螢光顯微鏡; 超磷酸化; ser-10位點磷酸化的組蛋白H3; 冷凍; 應急反應; Freezing; Hyperphospharylation; Immunofluorescence microscopy; Phosphorylation of histone H3 at serine 10; Stringent response; |
語 文 | 英文(English) |
中文摘要 | 植物對環境的改變會做出不同的反應。在本研究中,我們利用間接免疫螢光標記技術和共聚焦顯微鏡技術觀察到被冷凍處理的小麥根尖細胞在整個有絲分裂過程的不同相期中組蛋白H3的ser-10位點都處在超磷酸化狀態。在正常的小麥根尖細胞中組蛋白H3的ser-10位點的磷酸化現象出現在有絲分裂的前期,消失於分裂末期,在有絲分裂的中期和後期其主要定位在著絲粒區域。但是在冷凍的小麥根尖細胞中,在整個有絲分裂期中ser-10位點被磷酸化的組蛋白H3一直分佈在整條染色體上,甚至在胞質分裂期也沒有消失。實驗結果推斷,這種超磷酸化現象是植物在極端生長環境下的一種應急反應。 |
英文摘要 | Plants are able to produce various responses to environmental changes. In this report, we demonstrate that freezing of wheat root tips results in hyperphospharylation of Histone H3 at serine10. In normal conditions, the serine10 phosphorylation of histone H3 occurs at the condensed chromosomes at prophase and vanishes at telophase. The phosphorylated H3 is present mainly in the pericentromeric regions at metaphase and anaphase. However, in the frozen cells, the phosphorylation of histone H3 at serine10 is distributed throughout the chromosome arms at all of the phases during mitosis, even at interphase and cytokinesis. The results support the notion that hyperphospharylation of Histone H3 at serine10 is related to a stringent response. |
本系統中英文摘要資訊取自各篇刊載內容。