頁籤選單縮合
題名 | 體細胞核轉置複製荷蘭牛的端粒長度分析=Telomere Length Analysis of Cloned Holstein Cattle Produced by Somatic Cell Nuclear Transfer |
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作者 | 蕭振文; 蔡麗卿; 王君今; 陳裕信; 曲鳳翔; 劉振發; 李善男; 陳立人; | 書刊名 | 畜產研究 |
卷期 | 40:3 2007.09[民96.09] |
頁次 | 頁141-149 |
分類號 | 437.21 |
關鍵詞 | 體細胞核轉置; 複製; 端粒長度; 牛; Somatic cell nuclear transfer; SCNT; Cloning; Telomere length; Cattle; |
語文 | 中文(Chinese) |
中文摘要 | 本研究主要目的在分析畜產試驗所應用體細胞核轉置(somatic cell nuclear transfer, SCNT)技術生產的4頭複製荷蘭牛與同年齡同性別對照牛在2-3歲與3-4歲時的端粒(telomere)長度,以了解體細胞複製牛與正常牛隻端粒長度的變化。試驗自複製牛在年齡2-3歲與3-4歲時分別採集全血,經遠心分離收集白血球萃取基因組DNA,再應用測定套組完成端粒長度(terminal restriction fragment, TRF)分析。結果顯示,4頭複製牛在2至3歲時端粒長度分別17.03、17.92、17.92及17.19 kb,平均端粒長度17.52 ± 0.41 kb;而6頭同年齡同性別對照牛之端粒長度分別為17.79、17.96、17.86、17.03、17.37及17.18 kb,平均端粒長度17.53 ± 0.39 kb,兩者間無顯著差異。複製牛在3至4歲時端粒長度分別為17.65、17.31、17.82及17.13 kb,平均端粒長度17.48 ± 0.31 kb;而6頭同年齡同性別對照牛之端粒長度分別為18.76、15.04、19.47、17.59、17.31及18.62 kb,平均端粒長度17.80 ± 1.57 kb,兩者間亦無顯著差異。供核的耳朵成纖維細胞端粒長度則為17.07 kb,與複製牛比較無顯著差異。本研究顯示,應用SCNT技術生產的複製牛其端粒長度在基因再程式化的過程能回復正常,而與正常對照組牛隻沒有差異,並未因為利用成體供核體細細進行SCNT而導致複製牛端粒短化的現象。 |
英文摘要 | The purpose of this study was to determine the telomere length of somatic cell nuclear transfer (SCNT) cloned calves and their age-match normal counterparts. Genomic DNA samples were purified from whole blood of cloned animals and their control counterparts for terminal restriction fragment (TRF) analyses. The results shoed that the telomere lengths of four cloned calves at 2-3 years of age weres 17.03, 17.72, 17.92 and 17.19kb (mean TRF length = 17.52 ± 0.41 kb), respectively. The telomere lengths of six age-match individual at the same age were 17.79, 17.76, 78.86, 17.03, 17.37 and 7.18 kb (mean TRF length = 17.53 ± 0.39 kb), respectively. The telomere lengths of four cloned calves at 3-4 year of age were 17.65, 17.31, 17.82 and 17.13 kb (mean TRF length = 17.48 ± 0.31 kb). The telomere lengths of six age-match individual at the same age were 18.76, 15.04, 19.47, 17.59, 17.31 and 18.62 kb (mean TRF length = 17.80 ± 1.57 kb), respectively. There was no significant difference in TRF between coned and normal age-match animals. Our results suggest that the genomes of cloned embryos were successfully reprogrammed after cloning and therefore the telomere length could be restored to the level as their normal counterparts animals. |
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