查詢結果分析
來源資料
頁籤選單縮合
題名 | 以啟動子轉殖細胞株篩檢調節免疫力之中藥的模式建立=Construction of Promoters Based Immunity Screening System and Its Application on the Study of Chinese Traditional Herbs |
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作者 | 林璧鳳; Lin, Bi-fong; |
期刊 | 中醫藥年報 |
出版日期 | 20071000 |
卷期 | 25:2 2007.10[民96.10] |
頁次 | 頁225-251 |
分類號 | 414.51 |
語文 | chi |
關鍵詞 | 短暫轉染; RAW264.7巨噬細胞株; EL-4T細胞株; 中草藥; IFN-γ; NF-κB; Transfection; RAW264.7 macrophage cell line; EL-4T cell line; |
中文摘要 | 本研究試圖以系統性之篩選模式從日常飲食中之食材去找尋具有調節免疫反應之中草藥。研究策略乃以分配萃取進行22種中草藥材部份純化所得之各區分物,利用NF-κB啟動子及IFN-γ啟動子與報導基因(Luciferase)結合所建構之質體,分別短暫轉染(transient transfection)至RAW264.7巨噬細胞株、EL-4 T cell,進行抗發炎與免疫調節相關中草藥的快速篩選,並探討其促發炎介質NO、TNF-α、IL-6、PGE2與Th1細胞激素IL-2等分泌量,以作迅速篩選之工具,俾能利用篩選中草藥的抗發炎活性或影響免疫反應之研究,以期建立一個可供天然物、中草藥進行發炎反應研究之不同種類細胞實驗模式。結果顯示不同的萃取方法所萃得之分子極性不同,有不同的效果,其中以EtOAc層區分物同時對NF-κB及IFN-γ Luciferase activity皆具影響,其中桑寄生可同時對LPS/IFN-γ共同活化刺激下NF-κB Luciferase activity及Con A活化刺激下IFN-γ、Luciferase activity皆具顯著抑制作用(p<0.05)。反之,晉耆則同時促進NF-κB及IFN-γ Luciferase activity。當歸可抑制NF-κB及IFN-γ Luciferase activity,且其亦可顯著抑制IL-6、PGE2之分泌量;但對IFN-γ Luciferase activity不具影響。杜仲葉、板藍根可抑制IFN-γ Luciferase activity,但不影響NF-κB Luciferase activity。而穿心蓮皆不影響NF-κB及IFN-γ Luciferase activity。故桑寄生、晉耆、當歸、杜仲葉、板藍根、穿心蓮等中草藥材區分物可能含有具有抗發炎和調節免疫成份,值得進一步探討。 |
英文摘要 | The purpose of this project is to establish a screening system for dietary factors including Chinese Traditional herbs, which may exert regulatory effects on immune and inflammatory responses. The screening system is based on the mechanisms by which the component or extract from Chinese Traditional herbs can activate or suppress the promoter of inflammatory activator NF-κB, Th1 cytokine IFN-γ. The Scheme for the screening system is to construct plasmids containing reporter gene luciferase with promoter sequence either of NF-κB, IFN-γ. These reporter plasmids were transiently transfected RAW 264.7 cell and EL-4 T cell to test for luciferase activity induced or suppressed by various Chinese Traditional herbs. The methanol extract of these Chinese Traditional herbs was further fractionated with hexane, ethyl acetate (EtOAc). The results showed that each fraction extract of most of the Chinese Traditional herbs suppressed luciferase activity through NF-κB promoter in LPS+IFN-γ-activated RAW 264.7 cell. EtOAc fraction of Taxilli ramulus decreased in NF-κB and IFN-γ reporter gene luciferas activity. Astragali radix fraction increased luciferas activity. Therefore these herbs with opposite effects on NF-κB and IFN-γ activity will be used for further study to investigate NF-κB and IFN-γ activity on immune regulation. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。