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頁籤選單縮合
題 名 | 三黃瀉心湯濃縮方劑與水煎劑中活性指標成分相對生可用率之研究=Relative Bioavailability of Bioactive Constituents between Commercial Extract and Traditional Herbal Decoction of San Huang Shel Shin Tang |
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作 者 | 侯鈺琪; | 書刊名 | 中醫藥年報 |
卷 期 | 25:1 2007.10[民96.10] |
頁 次 | 頁1-65 |
專 輯 | 中醫藥療效評估之研究 |
分類號 | 413.95 |
關鍵詞 | 三黃瀉心湯; 濃縮方劑; 結合態代謝物; San Huang Shel Shin Tang; Conjugated metabolites; Bioavailability; |
語 文 | 中文(Chinese) |
中文摘要 | 目前臺灣中醫臨床上使用之中藥劑型主要為傳統水煎劑及濃縮製劑。鮮見有評估濃縮製劑與傳統水煎劑在療效上相等性之研究。本研究以三黃瀉心湯為模型,探討濃縮方劑與水煎方劑中活性指標成分相對生可用率,研究濃縮方劑是否可達與傳統水煎劑相等的口服吸收率。 本研究隨機收集市售GMP藥廠三黃瀉心湯濃縮散劑檢品11種,另依據標準劑湯方式製備三黃瀉心湯之水煎劑,並以高效液相層析儀(HPLC)建立定量兩類劑型中,其所含黃連鹼、棕櫚鹼、小檗鹼、黃芩苷、黃芩苷元、漢黃芩素、大黃素、蘆薈大黃素、大黃酸、大黃酚等指標成分含量之方法,結果顯示,各藥廠三黃瀉心湯濃縮散劑每克所含成分彼此差異甚大。 在大鼠口服兩種劑型之相對性可用率研究部分,我們以水煎劑為基準,投予動物相當於化學相當量之濃縮散劑,實驗採交叉設計,血清檢品於水解前及經sulfatase和ß-glucuronidase分別水解後,以自行開發之HPLC方法,定量各成分原型和結合態代謝物,藉以比較兩種劑型活性指標成分之生可用率,結果顯示,口服濃縮散劑組,除黃芩苷元外,蘆薈大黃素、漢黃芩素、大黃酸、大黃素、大黃酚等結合態代謝物之曲線下面積低於口服水煎劑分別達83.6%、42.2%、35.5%、46.0%、25.2%。總之,由本動物實驗血藥濃度之結果顯示,三黃瀉心湯水煎劑型比濃縮劑型有較佳之生可用率,此一現象顯示中藥濃縮製劑之賦型劑配方對其藥物指標成分之吸收應具一定程度之影響。 |
英文摘要 | The commercial extract of San Huang Shel Shin Tang (SHSST) in market were randomly collected in this study. The quantitation method of coptisine, plamatine, berberine, baicalin, baicalein, wogonin, emodin, aloe-emodin, rhein, chrysophanol using HPLC was developed and validated in this study. Samples were extracted by 70% methanol, and the aglycones and glycosides of each constituent were determined prior to and after hydrolysis. The results showed that the contents of the bioactive constituents in commercial extract of SHSST among manufactures were quite different. Rats were administered with traditional decoction and a commercial extract of SHSST in crossover design. The aglycones and conjugates metabolities were measured by HPLC prior to and after hydrolysis with sulfatase and ß-glucuronidase, respectively. The results showed that the AUC of the conjugated metabolites of aloe-emodin, wogonin, rhein and emodin from commercial extract were significantly lower than those from decoction by 83.6%, 42.2%, 35.5%, 46.0%, and 25.2% respectively. These results indicated that the dosage form of decoction confers better bioavalibility than the commercial extract of SHSST. |
本系統中英文摘要資訊取自各篇刊載內容。