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題名 | Effects of Retinoic Acid on Morula-stage Embryo Development in Mice=維他命A衍生老鼠桑椹期胚胎的影響 |
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作者姓名(中文) | 黃富仁; | 書刊名 | 長庚醫學 |
卷期 | 31:1 2008.01-02[民97.01-02] |
頁次 | 頁44-51 |
分類號 | 417.131 |
關鍵詞 | 維他命A衍生物; 小白鼠; 桑椹期胚胎; 胚胎發育學; Retinoic acid; Mice; Morula stage embryo; Embryology; |
語文 | 英文(English) |
中文摘要 | 背景:在我們的前期研究中,已顯示過量的維他命A衍生物作用在小鼠的桑椹期胚胎,並不會造成著床前囊胚期胚胎發育的負面影響。此研究將進一步探討維他命A衍生物,對桑椹期胚胎著床後胚胎發育的影響。 方法:在此前瞻性的小鼠體內研究,維他命A衍生物的給予劑量為0毫克/公斤與50毫克/公斤兩組;在第八天下午收集胚胎觀察並記錄結果。在小鼠的體外研究,收集桑椹期胚胎分別在不同維他命A衍生物濃度下(0, 0.001µ, 0.1µ, 10µM)作用一天後再培養七天,觀察胚胎發育並記錄結果。 結果:不同劑量的維他命A衍生物作用在桑椹胚胎,並不影響著床前囊胚期胚胎的形成。但它會影響囊胚期胚胎著床後的發育。 結論:此研究發現過量維他命A衍生物作用在小鼠桑椹期胚胎,雖然沒有造成胚胎短期傷害,卻造成了囊胚期胚胎著床後的長期傷害。 |
英文摘要 | Background: In a previous study, we investigated the short-term effects of in utero retinoic acid (RA) exposure on early development at the cleavage stage before implantation to understand the possible roles of RA in blastocyst formation. We designed the present study to investigate the long-term effects of RA exposure on pre-implantation embryos in vivo and in vitro. Method: To evaluate late pre-implantation exposure to RA, pregnant female mice were fed peanut oil with 50 mg/kg RA by oral gavage in the late afternoon of day 2 and early morning of day 3 of gestation. Mice were sacrificed on day 8. The number and morphology of embryos were recorded. The morula-stage embryos were treated with different doses of t-RA for 1 day and were cultured for the following 7 days in vitro. The post-implantation development in vitro was evaluated. Results: The in vivo study showed that the rate of blastocyst implantation was not significantly different (9.2 vs 10.2 per mouse) and the rate of post-implantation embryo resorption was significantly higher in retinoic acid treated mice than in controls (35% vs 0%). RA was administered to morula-stage embryos at levels of 0, 0.001μ, 0.1μ, or 10μ M throughout in vitro culture. For embryos that continued to develop following implantation, the development stages were delayed when assessed in 7-day culture. The percentage of embryos in the later stages of development changed depending on the dose of retinoic acid used. Conclusion: These findings document, for the first time, that RA exerts adverse effects on morula-stage embryos during the early stage of development. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。