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題名 | 甜菜夜蛾核多角體病毒噴霧感染甜菜夜蛾之效果評估=Assessment of the Efficacy of Aerosol Infection by Baculovirus on Spodoptera Exigua Larvae |
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作者 | 靳子蓉; 高穗生; 陳瀅如; 吳宗遠; | 書刊名 | 臺灣昆蟲 |
卷期 | 27:3 2007.09[民96.09] |
頁次 | 頁183-193 |
分類號 | 433.85 |
關鍵詞 | 核多角體病毒; 噴霧感染; 生物農藥; 甜菜夜蛾; 硼酸; Multiple nucleopolyhedrovirus; Spray infection; Bioinsecticide; Spodoptera; Boric acid; |
語文 | 中文(Chinese) |
中文摘要 | 於本研究中,我們證明加州苜蓿夜蛾核多角體病毒(Autographa californica multiple capsid nucleopolyhedrovirus, AcMNPV)可以噴霧的方式感染甜菜夜蛾幼蟲。進一步的研究顯示二齡甜菜夜蛾幼蟲對SpeiMNPV噴霧感染法之感受性較三或四齡甜菜夜蛾幼蟲為高;可達76%的感染率。除此之外我們也證實硼酸可以增強甜菜夜蛾幼蟲對SpeiMNPV噴霧感染法之感受性。因此我們進一步建立以甜菜夜蛾幼蟲蟲體噴霧感染法生產甜菜夜蛾核多角體病毒的方法,結果以三齡甜菜夜蛾幼蟲最適合以噴霧感染法生產甜菜夜蛾核多角體病毒之包含體。 |
英文摘要 | Spodoptera exigua multiple nucleopolyhedrovirus (SpeiMNPV) is a potentially promising biological control microbe. However, the generation of defective interfering particles (DI) when produced in a laboratory by insect cultures is an obstacle for the practical use of SpeiMNPV based insecticide. In this study, we established a convenient aerosol infection procedure for Spodoptera exigua larvae and occlusion body production by S. exigua larvae. We found that second instar S. exigua larvae were more accessible than third or fourth instar larvae for infection with SpeiMNPV budding virus using aerosol. Furthermore, 2% boric acid can enhance the efficacy of aerosol infection as well as the mortality for S. exigua larvae. We also demonstrated that the S. exigua third instar larvae were optimal for occlusion body production by aerosol infection. |
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