查詢結果分析
相關文獻
- Salt-modified Polycaprolactone Collagen Scaffold for Urothelial Cell Growth
- 組織工程中的膠原蛋白
- 牙科再生醫學與細胞組織工程之最近發展
- 顱顎障礙症與膠原蛋白異常之相關性
- 尿道注射膠原蛋白及其它充填劑治療應力性尿失禁
- Effect of Ascorbic Acid on Mouse Plasma Fibronectin Concentration
- 膠原蛋白膜於牙周再生的應用
- 豬皮中低終端冴膠原蛋白之製備
- 不同水煮時間對滷豬腱肉化學及流變性之影響
- Serum Antibodies to Native and Denatured Type I and III Collagen in Patients with Periodontal Disease
頁籤選單縮合
題名 | Salt-modified Polycaprolactone Collagen Scaffold for Urothelial Cell Growth=以塩化聚己內酯多元醇膠原蛋白作為尿路上皮細胞生長之生物鷹架 |
---|---|
作者 | 于大雄; 陳宏一; 張聖原; 馬正平; | 書刊名 | 臺灣外科醫學會雜誌 |
卷期 | 40:6 2007.11-12[民96.11-12] |
頁次 | 頁229-235 |
分類號 | 416.27 |
關鍵詞 | 塩化聚己內酯多元醇; 膠原蛋白; 尿路上皮細胞; 組織工程; Scaffold; Salt modidfied PCL-collagen copolymer; Urothelial cells; Tissue engineering; |
語文 | 英文(English) |
中文摘要 | 我們設計並合成聚己內酯多元醇生物鷹架聚合物,希望在施行組織工程治療中可增強它的細胞粘附力、孔洞及生物相容性。此塩化聚己內酯多元醇膠原蛋白聚合物乃利用不同重量比之塩分以二氧陸園聚合化方式合成並披覆上膠原蛋白。不同厚度之生物鷹架合成後並置於鐵氟龍板乾燥備用。合成之生物鷹架分別由形態、生物機械及生物相容性等屬性分析其特徵。細胞生長及形態乃利用倒立顯微鏡及掃瞄式電子顯微鏡觀察。培養細胞生長的速率乃以微培養tetrazolium方法測定,並利用特定細胞標誌染色來確認培養細胞之屬性。生物鷹架之生物分解力乃長期置放浸泡於培養液中觀察。結果顯示合成之聚己內酯多元醇膠原蛋白聚合物頗具彎曲度及彈性,並且在週期機械拉張測試下仍可維持其高張性。此多孔性生物鷹架可在培養液中維持原型至少一至三週而無明顯分解現象。當以1:4重量比塩化修飾此聚己內酯多元醇膠原蛋白生物鷹架時,我們發現可粘附生長之纖維細胞及尿路上皮細胞較之單純的聚己內酯多元醇明顯增加。這些細胞的粘附及生長可在二天間隔期下觀察到並持續增殖直到第三週。生物鷹架孔洞愈大時細胞的粘附及生長愈佳。由此研究驗證此塩化聚己內酯多元醇膠原蛋白生物鷹架可適於尿路上皮細胞之粘附生長。日後此生物鷹架對於泌尿組織工程之應用再造應有助益。 |
英文摘要 | Objective: A synthetic polymer, polycaprolactone (PCL) scaffold, was designed in order to enhance its cell affinity, porosity, and biocompatibility in performing tissue engineering. Methods: The salt-modified PCL-collagen copolymer was synthesized by a dioxane polymerization method with different weight ratio of salt and coated by collagen. Scaffolds with various thickness were synthesized and dried in a Teflon plate. The characteristics of these synthetic scaffolds were analyzed with regard to morphological, biomechanical and biocompatibilitic properties. Cellular growth and morphology were observed under an inverted microscope and a scanning electron microscope. The proliferation rate of cultured cells was determined using a microculture tetrazolium method. The confirmation of cultured cells was performed through several aspects using specific marker stains. The biodegradability of scaffolds was examined after long term soaking in the medium. Results: The copolymer was very flexible and elastic, and they could maintain their high elasticity under cyclic mechanical strain conditions. The highly porous scaffold withstood for an initial 1 week without any deformation and sustained for at least 3 weeks in culture media without prominent degradation. When 1:4 wt% salt was used to modify the PCL-collagen scaffold, the amount of adhesion of the fibroblasts and cultured urothelial cells on the scaffolds dramatically increased when compared with the control PLC alone. The taking and growth of cells were observed at 2 days interval and continued in proliferation of cells for 3 weeks. The cell adhesion and proliferation on the scaffolds of various pore-size were increased with increasing pore size. Conclusions: These results indicate that the salt-modified PCL-collagen copolymer scaffolds are feasible for urothelial proliferation. The improvement may be helpful for the extensive applications of PCL-salt-collagen copolymer scaffold in urological tissue engineering. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。