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題名 | 骨髓間葉幹細胞和PLA/PGA並用對骨大缺損組織再生之相關細胞蛋白表現的影響=Effects of Marrow Mesenchymal Stem Cells and PLA/PGA on Cellular Protein Expression during Regeneration of Large Bone Defects |
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作者姓名(中文) | 史中; 夏毅然; 彭志綱; 張燕清; | 書刊名 | 中華民國口腔顎面外科學會雜誌 |
卷期 | 18:2 2007.09[民96.09] |
頁次 | 頁81-88 |
分類號 | 368.53 |
關鍵詞 | 鹼性磷酸酶; 間葉幹細胞; 造骨細胞; 第一型膠原蛋白; Alkaline phosphatase; Mesenchymal stem cell; Osteoblast; Type I collagen; |
語文 | 中文(Chinese) |
中文摘要 | 日常生活中常因意外事故 (例:車禍等) 造成顎顏面或四肢區域骨折或骨最大缺損的發生。發生骨折後,輕微的會造成局部功能喪失,嚴重時甚至有生命之危險。所以維護骨組織及細胞的健全結構與功能是口腔顎面外科及急診醫學上重要的課題。 文獻回顧,有關利用骨髓幹細胞配合組織工程促進骨大缺損之癒合及組織再生時,相關細胞蛋白表現的實際改變情形並不清楚,但卻相當重要及值得去探討的。 本研究旨在利用申請人建立之骨髓細胞體外骨生成模式,自大白鼠脛骨和股骨中取出的骨髓細胞,經Ficoll-Paque比重梯度分離法所獲得含間葉 (或骨) 幹細胞之小比重骨髓細胞進行起細胞培養,並利用細胞標記 (CD marker) 分離出間葉幹細胞,將此骨髓間葉幹細胞 (MSCs) 先貼附在PLA/PGA生醫材料上再植入鼠齡為12個月成年大白鼠骨大缺損處,四週後利用細胞培養和細胞生物學相關技術 (相位差鏡檢、造骨細胞鹼性磷酸酶蛋白表現法和膠原蛋白表現法等) 評估骨髓間葉幹細胞和PLA/PGA並用防治和促進骨缺損修復/骨再生的效果及可能的細胞機轉。實驗結果發現在誘導因子處理後,可見造骨細胞分化、礦物化骨群體形成情形,表示分離出之間葉幹細胞具有骨生成分化能力。經MSCs及生醫材料植入,其鹼性磷酸酶蛋白表現在植入四週時有顯著增加趨 勢 (p<0.05,MSCs組與對照組比較;p<0.01,MSCs及生醫材料組與對照組比較)。另外,第一型膠原蛋白表現在MSCs、生醫材料或MSCs/生醫材料處理四週後,發現MSCs組及MSCs/生醫材料組明顯增加其表現 (p<0.05,與對照組比較)。因此,本研究結果顯示骨髓間葉幹細胞和PLA/PGA並用,可經由促進骨生成相關蛋白之表現而有效促進骨組織再生。 |
英文摘要 | The fractures and critical sized defects of bone in oral maxillofacial and other regions often occurs during daily life. A fracture could result in loss of function or even death. It is still not clear regarding the related protein expression during cell/tissue regeneration from unhealed fracture/bone defect. Thus, investigation on keeping bone tissue and cells functionally and prevention of damage of bone structure are imperative in oral and maxillofacial surgery and emergency medicine. The purpose of this study was to investigate the changes in cellular protein expressions during large bone defect regeneration after transplantation of stem cells and PLA/PGA using phase contrast microscopy, alkaline phosphatase protein expression, and type I collagen expression. Four weeks after treatment with MSCs and biomaterials, significantly increases in alkaline phosphatase protein and type I collagen protein expression were noted as compared to the controls. The results of this study indicated that a combination of MSCs and biomaterials significantly enhance bone defect regeneration at the protein level. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。