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頁籤選單縮合
題名 | 錦鯉疱疹病毒分離技術之建立=Development of Isolation Techniques on Koi Herpesvirus |
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作者 | 涂堅; 黃淑敏; 程建智; 張惟茗; | 書刊名 | 行政院農業委員會家畜衛生試驗所研究報告 |
卷期 | 41 民95.12 |
頁次 | 頁117-121 |
分類號 | 439.19 |
關鍵詞 | 錦鯉; 分離; 錦鯉疱疹病毒; Koi; Isolation; Koi herpesvirus; |
語文 | 中文(Chinese) |
中文摘要 | 本計畫目的為建立對錦鯉疱疹病毒有感受性之細胞株並發展本病毒之分離方法。我們共發展出兩種初代細胞株(鰭及鰓細胞,目前為35 代),生長溫度介於20-25oC,適當培養液為添加10% 胎牛血清的Leibovitz-15 (L-15),或添加10% 胎牛血清的60% Earle’s minimum essential medium (MEM)、10%tryptose phosphate 及20% L-15 之混合培養液。有關病毒感受性方面,培養在20oC,細胞病變在鰓細胞約10-14 天出現;在鰭細胞需20 天。典型細胞病變為細胞質內的空泡化及細胞溶解。使用鰓或鰭細胞分離本病毒,初代呈現細胞病變,但繼代則無細胞病變。 |
英文摘要 | The purpose of this project is establishing cell lines permissible to koi herpesvirus (KHV) for developing the isolation method of KHV. We established two primary cell lines from koi (35-passage koi gill and koi fin); the optimum growth medium was either Leibovitz-15 supplemented with 10% fetal calf serum or a mixture containing 60% Earle’s minimum essential medium, 30% Leibovitz-15, 10% tryptose phosphate and 10% fetal calf serum. The optimum growth temperature was between 20-25oC. Those two cell lines were susceptible to KHV and the cytopathic effect (CPE) appeared in koi gill cell 10-14 days post infection; and 20 days post infection in fin cell. The typical CPE showed cytoplasmic vacuolation and cell lysis. The CPE appeared only at first generation of isolation but it disappeared after the sub-passage of the virus in the same cells. |
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