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來源資料
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題 名 | 家禽流行性感冒核酸疫苗製備亞型特異性抗體之研發=Development of Avian Influenza Subtype-Specific Reference Antisera by DNA Vaccination |
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作 者 | 李敏旭; 陳麗旋; 劉玉彬; 鄭明珠; 李淑慧; | 書刊名 | 行政院農業委員會家畜衛生試驗所研究報告 |
卷 期 | 41 民95.12 |
頁 次 | 頁57-60 |
分類號 | 437.245 |
關鍵詞 | 家禽流行性感冒; DNA疫苗; 特異性抗體; Avian influenza; DNA vaccine; Specific antibody; |
語 文 | 中文(Chinese) |
中文摘要 | 將家禽流行性感冒病毒H5 基因之HA1 片段以RT-PCR 增幅出來後,將此基因轉殖到pCI-neo 及pCDNA3.1 載體,篩選好之真核表現載體配合微脂體以肌肉注射100 μg接種在6週齡BALB/c小鼠 及3週齡無特異病原小雞,之後每月補強接種共二次,經三次免疫後測試其抗體反應,結果顯示血球凝集抑制抗體反應均不佳。 |
英文摘要 | The HA1 subunit of the H5 gene was amplified by reverse transcriptase / polymerase chain reaction (RT-PCR) and cloned into the PCR-Ⅱ Blunt Topo vectors (Invitrogen ). Then the HA1 gene was subcloned into the pCI-neo vector(Promega,Madison, WI) and pCDNA3.1 vector (Invitrogen ). Six-week-old BALB/c mice and three-week-old specific pathogen free (SPF) chickens were vaccinated with 100 μg of eukaryotic expression vectors mixed with a liposome by intramuscular injection. Then the animals were boostered twice at monthly intervals after the first vaccination. The HI antibody titers were low. |
本系統中英文摘要資訊取自各篇刊載內容。