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頁籤選單縮合
題名 | 深層海水中乳酸菌的分離與鑑定=Isolation and Identification of Lactic Acid Bacteria from Deep Sea Water |
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作者 | 黃美瑩; 張錦宜; 張慎恩; 洪珮馨; 林金榮; | 書刊名 | 水產研究 |
卷期 | 15:1 2007.06[民96.06] |
頁次 | 頁45-54 |
分類號 | 369.35 |
關鍵詞 | 深層海水; 乳酸菌; 分離; 鑑定; Lactic acid bacteria; Deep sea water; Isolation; Identification; |
語文 | 中文(Chinese) |
中文摘要 | 本研究採集台灣東部知本外海不同水深 (285 ~ 912 m) 之深層海水樣品8件,水樣經濾膜過濾培養 (25℃,72小時) 後,於MRS Agar 上面共有35個菌落出現。經鑑定後,有2株具有乳酸菌屬的特性,該2株分別來自測站ST14 水深321 m 及530 m,編號為ST14-321 LA1 及ST14-530 LA1,其革蘭氏染色為陽性,均為四聯球菌,不具觸酶活性,為同性發酵。依據各項生理及生化特性,以及對於各種碳水化合物之利用性,將菌株歸屬為Pediococcus urinaeequi。該2株菌於不同溫度、pH與鹽度下之成長趨勢相當接近,於25℃與pH 8時生長最良好,鹽度為1 ~ 7% 時生長佳。雖然該2株菌其生理及生化特性相當接近,但對於碳水化合物之發酵利用情形則不太相同。 |
英文摘要 | Isolation and identification of lactic acid bacteria (LAB) from deep sea water were conducted in this study. Eight samples (from 285 m to 912 m) of deep sea water were collected from Chinpen (eastern Taiwan) off-shore. The samples were filtered through membranes (0.22 µm) and cultured on MRS agar at 25℃ for 72 hours. Among the 35 isolates, 2 isolates were identified as LAB. The 2 isolates, named ST14-321 LA1 and ST14-530 LA1, were sampled from the depth of 321 m and 530 m of the station 14, respectively. They were both Gram positive, cocci in tetrads, catalase negative, and homofermentative. Based on the physiological, biochemical characteristics and 16S rRNA sequence analysis, the 2 isolates were identified as Pediococcus urinaeequi. In addition, the results showed that optimum temperature, pH, and salinity for their growth were similar, which were 25℃, 8.0, and 1-7%, respectively. However, their fermentation profiles were different. |
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