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題 名 | 3-Aminobenzamide對過氧化氫誘發大鼠支氣管肺泡沖洗細胞之DNA單股斷裂之修復及細胞死亡形式之影響=Effects of 3-Aminobenzamide on the Repairing of DNA Single Strand Breaks and the Modes of Cell Death in Rat Bronchoalveloar Lavage Cells Exposed to Hydrogen Peroxide |
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作 者 | 陳永俐; 鄭穹翔; 李建穀; | 書刊名 | 臺灣獸醫學雜誌 |
卷 期 | 33:2 2007.06[民96.06] |
頁 次 | 頁114-122 |
分類號 | 437.22 |
關鍵詞 | DNA單股斷裂; 細胞凋亡; 壞死; PARP; 3-Aminobenzamide; DNA single strand break; Necrosis; Apoptosis; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究探討3-Aminobenzamide (3-AB), poly (ADP-ribose) polymerase (PARP),之專一性抑制劑,對於過氧化氫(H2O2)導致之大鼠支氣管肺泡沖洗細胞(Rat Bronchoalveloar lavage Cells) (RBALC)之細胞核DNA單股斷裂之修復,以及沖洗細胞之死亡形式之劑量及時間效應。應用單細胞電泳法檢測RBALC在H2O2於0-100 µM濃度範圍作用一小時後,DNA斷裂程度與濃度呈現正相關之現象(P<0.05)。添加3-AB並給予細胞3及6小時修復時間,顯示DNA斷裂之修復皆有顯著抑制(p<0.05)。以Trypan blue 排除試驗顯示,添加3-AB並與細胞共同培養修復3及6小時,則有顯著提高細胞在存活率之效果(p<0.05)。當以電泳檢測細胞DNA laddering pattern之形成以顯示細胞凋亡(apoptosis),3-AB之添加可將H2O2所造成之apoptosis之出現時間,由9小時縮減為3小時。形態學檢查則顯示3-AB之添加並同時給予12小時之修復,可降低細胞以壤死(necrosis)形式死亡之死亡率達25-30%。本研究結果顯示3-AB可能藉由抑制PARP活性,抑制RBALC DNA斷裂之修復,並可能將細胞死亡由壞死轉向成apoptosis之形式。 |
英文摘要 | This study was focused on the influences of 3-Aminobenzamide (3-AB), an inhibitor to poly (ADP-ribose) polymerase (PARP), on the DNA single strand break and repair, and the modes of cell death in rat Bronchoalveloar lavage cells (RBALC) following hydrogen peroxide (H2O2) exposure. By single cell gel electrophoresis assay, RBALC DNA single strand breaks were significantly increased as H2O2 concentration raised (p<0.05). Repairing of DNA single strand breaks were both significantly inhibited after addition of 3-AB for either 3 or 6 hours to RBALC that were previously exposed to H2O3 (p<0.05). Trypan blue excluding tests revealed significant increases in the survival rates of H2O2-treated RBALC after co-incubated with 3-AB for both 3 and 6 hours (p<0.05). Addition of 3-AB was also capable of shortening the onset of DNA laddering pattern (indication of apoptosis at early stage) by 6 hours and DNA laddering pattern peaked after 9 hours co-incubation with 3-AB. Morphologically, 3-AB did not switch cell death from necrosis to apoptosis; however, up to 30% of decrement in survived RBALC population could be observed after 12 hours repairing with 3-AB. These results suggested that 3-AB inhibited repairing of DNA single strand breaks induced by H2O2, it also might postpone cellular necrosis and redirected the cells to apoptosis by inhibiting PARP activities. |
本系統中英文摘要資訊取自各篇刊載內容。