查詢結果分析
來源資料
頁籤選單縮合
題名 | 澳洲黑天鵝性別鑑定遺傳標記=Genetic Marker for Sex Identification of Australian Black Swan |
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作者 | 林德育; 劉瑞珍; 陳若菁; 吳國欽; 張秀鑾; 吳明哲; | 書刊名 | 畜產研究 |
卷期 | 39:4 民95.12 |
頁次 | 頁281-288 |
分類號 | 437.75 |
關鍵詞 | 澳洲黑天鵝; 逢機複製多態性DNA; 性別鑑定; Black swan; Random amplified polymorphic DNA; RAPD; Sexing; |
語文 | 中文(Chinese) |
中文摘要 | 澳洲黑鵝性別為一性單型物種(monomorphic species),從外觀不易分辨出雄雌性別,為尋求澳洲黑天鵝性別遺傳標記,本研究應用逢機複製多態性DNA(random amplified polymorphic DNA, RAPD)分析方法,行政院農業委員會畜產試驗所彰化種畜繁殖場所飼育的澳洲黑天鵝個體全血DNA進行性別遺傳標記探討。第一階段以6公6母澳洲黑天鵝個體DNA作為模板,共作用220組寡核苷酸RAPD引子進行分析,尋找可區分性別的候選遺傳標記。分析結果顯示220組寡核苷酸引子中,引子組AE03(5'-CATAgAgCgg-3')所產出之PCR產物中的670 bp DNA片段為雌性澳洲黑天鵝特有的片段,可作為區分別性別的標記。第二階段,再以引子組AE03分析該場全場共58隻(28公30母)澳洲黑天鵝之DNA樣本,檢測結果顯示由引子組AE03分析DNA判讀之性別均與實際性別完全吻合,準確率為100%(58/58);意即所有檢測雌性澳洲黑天鵝PCR產物DNA片段中均具有670 bp DNA片段,而在所有受檢雄性澳洲黑天鵝則無,證實RAPD引子組AE03可作為區分性別之遺傳標記。 |
英文摘要 | To search for a sex identification candidate marker for the Australian black swan, a sexually monomorphic species, whole blood DNAs of individual Australian black swan (from Changhua Animal Propagation Station of Livestock Research Institute, Council of Agriculture) were analyzed by random amplified polymorphic DNA (RAPD). In phase I, 220 different 10-mer primers of arbitrary sequence were used to screen twelve Australian black swan (6 males and 6 females). An RAPD marker (AE03, 5'-CATAgAgCgg-3') demonstrated high sensitivity and accuracy in gender diagnosis. A sex-specific DNA fragment (670 bp) amplified with primer AE03 could be observed in the RAPD fingerprints of females only. The proof stage, phase II, AE03 was used to test other 58 birds (28 males and 30 females) from the Station in Sep. 2001. Results showed that it was 100% (58/58) accurate. The specific DNA fragment (670 bp) amplified with primer AE03 could therefore be a sex identification marker of the black swan. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。