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題 名 | Endothelin-1 Antisense Oligonucleotide Suppresses the Proliferation of Glomerular Mesangial Cells Stimulated with Angiotensin-II=內皮素-1反譯寡核苷酸抑制第二型血管收縮素所誘發之腎絲球間質細胞增生 |
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作 者 | 李佳蓉; 辛錫璋; 邱怡文; 陳鴻鈞; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 23:4 2007.04[民96.04] |
頁 次 | 頁170-175 |
分類號 | 415.74 |
關鍵詞 | 第二型血管收縮素; 反譯寡核苷酸; 內皮素-1; 腎絲球間質細胞; Angiotensin-II; Antisense oligonucleotide; Endothelin-1; Mesangial cell; |
語 文 | 英文(English) |
中文摘要 | 反譯寡核苷酸 (以下簡稱 AON) 最近被報告可能做為臨床治療用途。我們曾報告第二型血管收縮素 (以下簡稱 Ang-II),會刺激另一個更強的細胞激素,內皮素-1 (以下簡稱 ET-1) 之產生,因此本研究之目的即探討 ET-1 AON 對 Ang-II 所誘發之腎絲球間質細胞增生之影響。ET-1 係以放射免疫分析法測定。研究結果 (1) Ang-II 可誘發腎絲球間質細胞產製較多量 ET-1 (2) ET-1 AON 可抑制腎絲球間質細胞 ET-1 之產量,而 ET-1 sense 及 scramble 寡核苷酸則沒有類似作用 (3) Ang-II 可刺激腎絲球間質細胞增加 thymidine 攝取量,此增加可受 ET-1 AON 部分抑制,但 ET-1 sense 及 scramble 寡核苷酸則沒有類似作用。本研究結果顯示 ET-1 AON 可明顯抑制腎絲球間質細胞之 ET-1 產量及 Ang-II 所誘發之細胞增生。 |
英文摘要 | Antisense oligonucleotide (AON) has been applied to modern molecular pharmacology. We have previously demonstrated that angiotensin-II (Ang-II) is an active stimulator of endothelin-1 (ET-1) production in glomerular mesangial cells. This study was designed to investigate the specific effect of ET-1 AON on inducing proliferation of cultured rat mesangial cells stimulated with Ang-II. ET-1 was measured by radioimmunoassays. The results were: (1) Ang-II enhanced ET-1 production of mesangial cells; (2) ET-1 production of mesangial cells was significantly suppressed by ET-1 AON, and this production was not affected by either ET-1 sense or scramble oligonucleotide in different concentrations; (3) Ang-II increased [3H]-thymidine uptake of mesangial cells, which was suppressed to 25% by ET-1 AON but not by ET-1 sense or scramble oligonucleotide. Our results indicate that ET-1 AON effectively suppresses the ET-1 production and the Ang-II-stimulated proliferation of mesangial cells, and therefore may offer treatment for proliferative glomerulonephritis. |
本系統中英文摘要資訊取自各篇刊載內容。